RAS基因多态性与子痫前期肾脏损害的相关性研究  

Study on the relationship between the RAS gene polymorphisms and renal impact in preeclampsia patients.

作　　者：李桦[1] 马玉燕[1] 王磊一[1] 

机构地区：[1]山东大学齐鲁医院,济南250012

出　　处：《中国优生与遗传杂志》2006年第11期11-13,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨肾素-血管紧张素系统基因多态性与子痫前期患者肾脏损害的相关性。方法随机选择子痫前期患者72例,正常妊娠妇女45例,利用PCR和RFLP反应分析其外周血白细胞中ACE基因和AT1R1166基因多态性,比较不同基因型子痫前期患者蛋白尿含量和血清肾功能指标的差异。结果1.ACE基因多态性与尿蛋白含量密切相关,携带D等位基因孕妇的尿蛋白含量显著增加,血清肌酐、尿素氮水平呈上升趋势。2.ACE基因和AT1R1166基因型分布频率在子痫前期患者与正常孕妇间无显著差异。结论ACE基因与子痫前期肾脏损害密切相关,D等位基因是导致肾脏损害的不利因素。Objective： To investigate whether the polymorphisms of angiotensin -converting enzyme （ACE） gene and angiotensin Ⅱ receptor type 1 （ AT1 R） gene were associated with renal impact in preeclampsia patients. Methods ： A case - control study was conduced including 72 PE patients and 45 normal pregnancies, The polymorphisms of ACE gene and AT1R^1166 gene were determined by polymerase chain reaction （PCR） and restriction fragment length polymorphism （RFLP）, The level of proteinuria, plasma creatinine, urea nitrogen and uric acid were compared by the genotypes, Results ： 1, There was significant difference in the level of proteinuria in different genotypes of ACE gene. DD and ID genotype was predominated in passive proteinuria, comparatively, Ⅱ predominated in mild proteinuria. The frequency of allele D was significantly higher in passive proteinuria. The trend of the level of plasma creatinine and nitrogen was DD 〉 ID 〉 Ⅱ. 2. No significant difference was found in the level of proteinuria, plasma creatinine and urea nitrogen among different genotypes in PE patients. 3. No significant difference in the allele and genotypes of the two genes was found between the PE patients and the controls. Conclusion： The two kinds of gene polymorphisms seemed associated with the severity of renal impact in PE patients, ACE D allele seems closely related with proteinuria. But No evidence was found in this study for the association of ACE L/D polymorphisms and AT1R polymorphisms with PE. These findings deserves to be further evaluated in larger studies, if positive, they will provide an additional marker for risk assessment of PE patients.

关 键 词：子痫前期 基因 多态性 血管紧张素转换酶 血管紧张素受体 

分 类 号：R714.2[医药卫生—妇产科学]