缺氧对肺动脉平滑肌细胞钾通道Kv1.2、Kv1.6基因表达的影响  

Effects of Hypoxia on Expressions of Voltage-gated K^+ Channels Kv1.2,Kv1.6 in Pulmonary Artery Smooth Muscle Cells

作　　者：廖文慧[1] 曾锐[2] 刘晓晴[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院综合科,邮政编码武汉430030 [2]华中科技大学同济医学院附属同济医院肾内科,邮政编码武汉430030

出　　处：《微循环学杂志》2006年第4期26-29,共4页Chinese Journal of Microcirculation

摘　　要：目的:研究不同缺氧时间对大鼠肺动脉平滑肌细胞(PASMC)不同亚型钾通道(Kv)mRNA和蛋白质表达的影响。方法:采用半定量RT-PCR和Western-blot方法对常氧和不同缺氧时间后PASMC上Kv1.2、Kv1.6mRNA和蛋白质的表达进行测定。结果:(1)PASMC在常氧和缺氧时均有Kv1.2、Kv1.6mRNA和蛋白质表达;(2)缺氧18h使Kv1.2mRNA和蛋白质表达增强,缺氧48h则使其表达减弱且低于常氧时的表达;(3)缺氧18h、48h对Kv1.6的mRNA和蛋白质表达无影响。结论:Kv1.6不是氧敏感的钾通道;作为氧感受器的Kv1.2,其mRNA和蛋白质表达随缺氧时间而改变。Objective: To investigate the effects of hypoxia on the expressions of mRNA and protein of voltage-gated K~ + channels in Pulmonary Artery Smooth Muscle Cells(PASMCs).Method: Reverse transcriptase-polymerase Chain Reaction (RT-PCR) and western-blot were used to determine the mRNA levels of Kv1.2 and Kv1.6 in normoxic and hypoxic PASMCs respectively.Western blot was used to determine the protein levels of Kv1.2 and Kv1.6 in normoxic and hypoxic PASMCs.Results: (1)The expressions of Kv1.2 and Kv1.6 were demonstrated both in normoxic and hypoxic cultured PASMCs by using RT-PCR and Western blot.(2)Eighteen hour-hypoxia increased while forty-eight hour-hypoxia decreased the expressions of Kv1.2 mRNA and protein significantly compared with normoxic group.(3)In contrast to Kv1.2,hypoxia negatively affected the expressions of Kv1.6 mRNA and protein in PASMCs.Conclusion: Kv1.6 acts insensitively to hypoxia.The expression of Kv1.2 in hypoxic PASMCs varies with the time of hypoxia.

关 键 词：平滑肌细胞钾通道 肺动脉高压 基因表达 缺氧环境 Pulmonary 低氧性肺血管收缩 Potassium 肺动脉平滑肌细胞 

分 类 号：R562.25[医药卫生—呼吸系统]