多波长高效液相色谱法同时测定黄连解毒汤中3类成分  

Determination of 3 kinds of components in Huanglian Jiedu decoction by HPLC under different UV wavelengths

作　　者：李新中[1] 雷鹏[1] 刘韶[1] 

机构地区：[1]中南大学湘雅医院,湖南长沙410008

出　　处：《中国中药杂志》2006年第20期1686-1688,共3页China Journal of Chinese Materia Medica

基　　金：湖南省卫生厅中医药科技基金(221201)

摘　　要：目的:同时测定黄连解毒汤中3类有效成分(盐酸小檗碱、盐酸巴马亭、盐酸药根碱、黄芩苷、栀子苷)的含量。方法:三波长同时检测的高效液相色谱法;D iamonsil C18色谱柱(4.6 mm×250 mm,5μm);流动相水-甲醇-0.05%磷酸(梯度洗脱);柱温35℃;流速1 mL.m in-1;检测波长345,280,238 nm。结果:建立了同时对黄连解毒汤中的5个成分进行定量的测定方法,本法快速、重复性好、灵敏度高。结论:为黄连解毒汤提供了更合理、可靠的质控方法。Objective： To determinate 3 kinds of effective components （5 compounds） in Huanglian Jiedu decoction at the same time, namely berberine, palmtine, jatrorrhizine, baiealin and geniposide. Method： A HPLC method detected by 3 different UV wavelengths 345 nm for berberine, palmtine and jatrorrhizine, 280 nm for baiealin, 238 nm for geniposide. Diamonsil C18 （4.6 mm × 250 mm, 5 μm） column was used, the mixture of H20 （A） , CHs OH （B） and 0. 05% Hs PO4 solution （C） as mobile phase in gradient mode. The column temperature was 35 ~C and the flow rate was 1 mL · min^-1. Result： This method was applied to determined 5 compounds in Huanglian Jiedu decoction rapidly and accurately. Conclusion： The quality control method is convenient, reasonable and credible for Huanglian Jiedu decoction.

关 键 词：高效液相色谱法 黄连解毒汤 盐酸小檗碱 盐酸巴马亭 盐酸药根碱 黄芩苷 栀子苷 

分 类 号：R284.1[医药卫生—中药学]