用^(41)Ca-AMS法测定谷氨酸毒性对PC12细胞外钙内流的影响  

Detecting the effects of glutamate toxicity on influxing calcium in PC12 cells by AMS

作　　者：袁媛[1] 李世红[1] 张东正[2] 何明[1] 董克君[1] 张根发[3] 姜山[1] 

机构地区：[1]中国原子能科学研究院 [2]上海交通大学物理系,上海200030 [3]北京师范大学生命科学院,北京100875

出　　处：《核技术》2006年第11期821-825,共5页Nuclear Techniques

基　　金：国家自然科学基金项目(10405036)资助

摘　　要：本工作用41Ca示踪PC12细胞,以CaF2作为最终测量样品,用加速器质谱计(全称AMS)测量41Ca。从谷氨酸毒性对PC12细胞的时间效应,MK-801对PC12细胞受体门控钙通道(全称ROC)阻断效应两方面进行分析,以激光扫描共聚焦显微镜的检测结果作为参照,结果表明41Ca的加速器质谱(AMS)测量方法能有效地检测到在不同情况下谷氨酸引起PC12细胞中外钙内流的情况。综合两种方法推测在谷氨酸引起的钙超载中,NMDAR介导的外钙内流起了重要作用。The PC12 cells were traced by ^41Ca and the final biological sample, CaFE, was measured by Accelerator Mass Spectrometry （AMS）. The results were analyzed from two aspects： the time effect of glutamate on free calcium in PC12 cells; and the blocking effect of antagonist, MK-801, on receptor operated channel （ROC）. Comparing with the results detected by laser scanning confocal microscope （LSCM）, the results show that ^41Ca AMS measurement can be used for detecting the change of calcium influx in PC12 cells effectively in different situations.

关 键 词：^41Ca 加速器质谱计 激光扫描共聚焦显微镜 PC12细胞 谷氨酸 MK-801 

分 类 号：O57[理学—粒子物理与原子核物理]