解脲脲原体垂直传播动物模型的建立  

The establishment of animal model by ureaplasma urealyticum vertical transmission

作　　者：吕杰[1] 李凤云[1] 唐素兰[1] 

机构地区：[1]蚌埠医学院微生物学教研室

出　　处：《蚌埠医学院学报》2006年第6期567-569,F0004,共4页Journal of Bengbu Medical College

基　　金：安徽省教育厅自然科学研究计划项目资助(2004kj274)

摘　　要：目的:建立妊娠期解脲脲原体(ureaplasma urealyticum,UU)生殖道感染垂直传播的动物模型。方法:血清4型UU标准株生殖道感染妊娠期昆明鼠,通过UU病原学检查、免疫组化等技术,观察妊娠期UU感染的垂直传播情况。结果:3.5×104CCU/ml、5×105CCU/ml、106CCU/ml UU感染的新生仔鼠肺、脾组织UU阳性率分别为0、0;2.7%、1.4%;74.4%、30.3%。结论:通过UU生殖道感染妊娠期孕鼠,成功构建妊娠期UU垂直传播的实验动物模型。Objective：To set up a vertical transmission animal model by genital infection with ureaplasrna urealyticum（ UU ） during the course of cyesls. Methods ： Kunming mice were infected by serotype 4 UU during the period of pregnancy through vagina, the vertical transmission from mother to filial generation was observed by UU culture and immumohistochemical staining test. Results ： UU positive rates from the lung and spleen in the newborn mice by UU irffection（3.5 × 10^4 CCU/ml,5 × 10^5 CCU/ml,and 10^6 CCU/ml） were 0 and 0,2.7% and 1.4% , and 74.4% and 30.3% , respectively. Conclusions：The UU vertical transmission animal model during the course of cyesis was constructed successfaly through genital infection with UU in the pregnant mice.

关 键 词：解脲脲原体 垂直传播动物模型 小鼠 实验 建立 

分 类 号：R375[医药卫生—病原生物学]