碱性单细胞凝胶电泳技术用于辐射损伤快速剂量估算的时效和量效关系研究  被引量:10

STUDY ON RELATIONSHIPS OF TIME-RESPONSE AND DOSE-RESPONSE OF RADIATION-INDUCED DNA DAMAGES USING SINGLE CELL GEL ELECTROPHORESIS FOR RAPID DOSE ASSESSMENT

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作  者:蒋亚齐[1] 陈英[2] 刘芬菊[1] 李友[2] 

机构地区:[1]苏州大学放射医学与公共卫生学院,215123 [2]军事医学科学院放射与辐射医学研究所,北京100850

出  处:《辐射防护》2006年第6期353-357,共5页Radiation Protection

摘  要:应用碱性单细胞凝胶电泳技术(SCGE),采用小鼠整体照射和人外周血离体照射方式,检测受60Co-γ射线照射的外周血细胞DNA损伤修复的时间效应和剂量效应关系,探讨SCGE用于辐射损伤快速剂量估算的可行性。整体实验结果表明,小鼠外周血细胞DNA损伤程度在照射后即刻最明显,照射后2h DNA损伤已基本修复,照射后24 h与照射前相比已无显著性差异(p>0.05)。离体实验结果发现,照射后即刻人外周血细胞尾矩TM值随剂量增加而增加,照射组与未照射组之间有非常显著性差异(p<0.01),剂量效应呈现良好的线性关系Y=0.3619+2.1834D(r=0.9946);人外周血细胞尾矩TM照射后6 h与未照射组相比已无显著性差异(p>0.05)。以上结果表明,碱性单细胞凝胶电泳是一种快速、灵敏检测DNA损伤的有效方法,具有良好的剂量效应关系;但修复较快,可测时间范围有限。The relationships of time-response and dose-response of DNA damages of peripheral blood cell induced by 60 Co T radiation were studied using single cell gel electrophoresis and the feasibility of SCGE as a biological dosimeter was discussed. Mice and human peripheral blood cells were irradiated in vivo and in vitro respectively, for drawing up curves of time-response and dose-response. In vivo, DNA damages in mice peripheral blood cells were the most serious immediately after irradiation, and were essentially repaired 2 h after irradiation and had no statistically significant difference (p 〉 0.05) between irradiation and control groups 24 h after irradiation. In vitro, TM value of human peripheral blood cells increased in a dose dependent manner, and a extremely statistically significant difference was observed between irradiation and control groups after irradiation immediately. The dose-response relationship well fitted with linear model: Y = 0. 3619 + 2. 1834D( r = 0. 9946). However, there has been no statistical difference (p 〉 0. 05 ) between irradiation and control groups 6 h after irradiation. The results indicate that SCGE is a rapid, sensitive method for examining radi- ation-induced DNA damages, and has showed a well lineal" dose-response relation although it can only measure in a limited time range as damages repaired more rapidly.

关 键 词:碱性 单细胞凝胶电泳 电离辐射 时间效应 剂量效应 

分 类 号:R144[医药卫生—公共卫生与预防医学]

 

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