黄芪注射液对雌激素受体的激活作用研究  

Activating Effect of Astragalus Injection on Estrogen Receptor

作　　者：王海彬[1] 王军舰[2] 黄辉[2] 刘琳[2] 刘少军[1] 徐传毅[1] 何伟[1] 樊粤光[1] 黄志伟[2] 

机构地区：[1]广州中医药大学第一附属医院,广东广州510405 [2]中国科学院广州生物医药与健康研究院,广东广州510663

出　　处：《时珍国医国药》2006年第11期2113-2115,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金资助项目(No.30400606);广东省自然科学基金资助项目(No.04010036);广东省科技厅科研项目(No.粤科社字2004;139号);广州中医药大学校创新基金资助项目(No.K004044)

摘　　要：目的构建含有雌激素受体配体结合域的哺乳动物细胞杂交基因报告系统,研究黄芪注射液对雌激素受体(ER)活性的影响。方法PCR扩增雌激素受体配体结合域,将配体结合域克隆到真核表达质粒pCMV-BD构建细胞杂交基因报告系统,研究黄芪注射液与雌激素受体的关系;MTT法检测黄芪注射液对细胞活力的影响。结果成功构建含有雌激素受体配体结合域的哺乳动物细胞杂交基因报告系统,黄芪注射液不能提高细胞活性,但对雌激素受体具有激活作用。结论黄芪注射液可能通过雌激素受体发挥疗效,细胞杂交基因报告系统是研究中药作用机理的手段之一。Objective To establish the Gal4DBD - ER or ER-LBD report system to study the effect of Astragalus Injection on ERs. Methods Gene report system was generated by amplifying the ligand binding domain（ LBD ） with Pfu polymerase and subcloning the product into the eukaryotie expression vector pCMV-BD. This system was employed to study the relationship between Astragalus Injection and ERs. Cell activity were detected by MTT. Results Gene reporter system using ER - LBD is established. Astragalus Injection can not improve cell activity but can activate ERs activation. Conclusion The mechanisms of Astragalus Injection may be related to its effect of activating ERs. Gene report system can be used to study the mechanisms of Chinese traditional medicine.

关 键 词：黄芪注射液 基因报告系统 雌激素受体 HELA细胞 配体结合域 

分 类 号：R285.5[医药卫生—中药学]