高效液相色谱法同时测定苦参片中苦参碱和氧化苦参碱的含量  

Determination of Oxymatrine and Matrine in Kushen Tablets by HPLC

作　　者：姜艳玲[1] 张善玉[1] 吕惠子[1] 

机构地区：[1]吉林省延边大学药学院,吉林延吉133000

出　　处：《时珍国医国药》2006年第11期2204-2205,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的建立苦参片中氧化苦参碱和苦参碱的含量测定方法。方法采用高效液相色谱法，使用Thermo色谱柱，以乙腈-磷酸盐缓冲液（取磷酸二氢钾1．36g，加1ml三乙胺，加水至1000ml，用磷酸调节pH5．5）（3：97）为流动相，流速为1．0mE／min，检测波长为220nm。结果氧化苦参碱在0．0055～0．088mg／ml之间与峰面积积分值呈良好的线性关系（r=0．9998），平均加样回收率99．41％，RSD为1．28％；苦参碱在0．106～0．170mg／ml之间与峰面积积分值呈良好的线性关系（r=0．9999），平均加样回收率99．35％，RSD=1．25％。结论该法灵敏、准确、专属性强，重现性好，可作为苦参片的质量控制方法。Objective To establish a method for simultaneous determination of the content of matrine and oxymatrine in Kushen Tablets. Methods HPLC method was carried out through Thermo C18 column, using acetontrile - phosphate buffer solution （contained 1.36 g potassium dihydrogen phosphate and 1 ml triethylarmine, add water to 1000 ml, adjusted to pH 5.5 with phosphate acid） （3： 97） as mobile phase. The flow rate was 1.0 ml/min with detective wavelength of 220 nm. Results The calibration curve of oxymatfine content was linear within the range of 0. 0055 - 0. 088 mg/ml （ r = 0. 999 8 ）, the average recovery of oxymatrine was 99.41% , RSD was 1.28% ;the calibration curve of matrine content was linear within the range of 0. 170 -0. 0106 mg/ml （r = 0.999 9） , the average recovery of Matrine was 99.35% , RSD was 1.25%. Conclusion The method is simple, fast and accurate, it can increase the quality control index of Kushen Tablets.

关 键 词：苦参片 氧化苦参碱 苦参碱 高效液相色谱 

分 类 号：R284.2[医药卫生—中药学]