构建人乳头瘤病毒关键基因的原核重组载体  

Prokaryotic recombination vector construction of HPV important gene

作　　者：潘巍巍[1] 赖国旗[2] 宋方洲[1] 易发平[3] 马永平[4] 左国伟[2] 

机构地区：[1]重庆医科大学分子生物学教研室,重庆400016 [2]重庆医科大学动物实验中心,重庆400016 [3]重庆市生物化学与分子药理学重点实验室,重庆400016 [4]教育部临床检验诊断学重点实验室,重庆400016

出　　处：《中国现代医学杂志》2006年第21期3201-3204,共4页China Journal of Modern Medicine

基　　金：国家自然科学基金资助项目(30371479);重庆医科大学基金;重庆市教委资助项目(渝教科[2005]12号);教育部"春晖计划"资助项目(Z2004-1-55103)

摘　　要：目的构建HPV16E6/E7关键基因的原核载体pET-32(+)-E6/E7并将其转到大肠杆菌BL-21(DE3)中诱导表达,最后获得E6/E7基因表达产物。方法用PCR方法从含有HPV16全基因序列的质粒中扩增E6/E7基因,将E6/E7基因连接到pET-32a(+)质粒上,并转到大肠杆菌BL21(DE3)中。最后检测E6/E7基因。结果获得重组后的载体pET-32(+)-E6/E7。结论成功构建表达了含有E6E7的原核重组载体,并在大肠杆菌中表达。[Objective] To construct prokaryotic vector of HPV16 E6/E7 important gene, then transform it into BL-21（DE3） E.coli for expression and obtain the expressive production of HPVE6/E7. [Methods] E6/E7 gene was amplified by polymerase chain reaction （PCR） from vector that contains all sequence of HPV16, the amplified fragnent E6/E7 gene was ligated into pET-32a （＋） vector and transformed into BL-21 （DE3）E.coli. E6/E7 gene was identified. [Result] We obtained recombinant pET-32（＋）-E6/E7 vector. [Conclusions] We have successfully constructed vectors that have prokaryotic recombinant vector of E6/E7 gene which has been expressed in E.coli..

关 键 词：人乳头瘤病毒 HPV16 E6/E7构建 重组载体 

分 类 号：R373.39[医药卫生—病原生物学]