鲍曼不动杆菌TEM-1型ESBLs基因及其多重耐药的研究  

Study on ESBLs gene of TEM-1 type and multi-drug resistance of Acinetobacter baumannii

作　　者：李文林[1] 李蓉[2] 石小玉[3] 梁朝[1] 赵林[1] 

机构地区：[1]江西省南昌大学医学科学研究所,江西南昌330006 [2]江西省南昌大学医学院病原生物学教研室,江西南昌330006 [3]江西省南昌大学医学生物高技术重点实验室,江西南昌330006

出　　处：《中国现代医学杂志》2006年第21期3257-3260,共4页China Journal of Modern Medicine

基　　金：江西省卫生厅科技项目(20043143)

摘　　要：目的分析产TEM-1型超广谱β-内酰胺酶(ESBLs)鲍曼不动杆菌的耐药性及其基因序列。方法用聚合酶链式反应(PCR)扩增产ESBLs鲍曼不动杆菌的TEM-1型耐药基因,用克隆测序方法分析质粒上TEM-1型ESBLs基因。结果15株产ESBLs鲍曼不动杆菌中,TEM-1型ESBLs阳性菌株有12株,TA克隆后测序表明,与鲍曼不动杆菌(AY560328.1)blaTEM-1基因序列99%同源。结论质粒上带产TEM-1型ESBLs的鲍曼不动杆菌,blaTEM-1基因与鲍曼不动杆菌多重耐药密切相关。[Obejective] To analyse drug resistance of Acinetobacter baumannii which produces TEM-1 ESBLs and its nucleotide sequence. [Methods] TEM-1 ESBLs on plasmid of ESBLs producing strains were analysed by PCR and nucleotide sequencing. [Results] 12 TEM-1 ESBLs producing strains were identified in 15 isolates of ESBLs （＋） Acinetobacter baumannii. Sequence alignmem analysis of this product revealed 99% identity with blaTEM-1 which was originally detected in another strain of Acinetobacter baumannii （AY560328.1）. [Conclusion] An Acinetobacter baumannii which harbors TEM-1 type ESBLs is detected. BlaTEM-1 gene is closely associated with MDR of Acinetobacter baumannii.

关 键 词：多重耐药(MDR) 鲍曼不动杆菌 超广谱β-内酰胺酶(ESBLs) 基因 

分 类 号：R378[医药卫生—病原生物学]