H_2O_2对大鼠肺动脉平滑肌细胞Kv通道的作用  

Effect of H_2O_2 on voltage-gated potassium channels in rat pulmonary artery smooth muscle cells

作　　者：骆红艳[1] 唐明[1] 杜以梅[1] 刘长金[1] 唐碧[1] 胡新武[1] 胡谋先[1] 席姣娅[1] 宋元龙[1] Jurgen Hescheler 

机构地区：[1]华中科技大学同济医学院生理系 [2]Institute of Neurophysiology,University of Cologne,D-50931 Cologne,Germany

出　　处：《中国病理生理杂志》2006年第11期2098-2101,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30200098)

摘　　要：目的:观察H2O2在常氧时对大鼠肺动脉平滑肌细胞(PASMCs)的Kv的影响,探讨H2O2对肺动脉平滑肌细胞的Kv通道的作用。方法:用酶解法急性分离单个PASMCs,以全细胞膜片钳技术记录PASMCs膜上的电压依赖性钾通道(voltage-gated potassium channel,Kv)电流。结果:常氧下H2O2可显著增加Kv电流,电流-电压关系曲线左上移;而且Kv电流呈浓度依赖性增加。结论:常氧下H2O2可使Kv通道开放。AIM： To determine the effect of hydrogen peroxide （H202 ） on voltage -gated potassium channel currents （IKv） in pulmonary vascular smooth muscle cells （PASMCs）. METHODS ： Using whole cell patch - clamp technique, IKv was recorded in freshly isolated rat PASMCs with acute enzymatic digestion method. The effect of hydrogen peroxide on IKv in PASMCs was investigated in normoxia. RESULTS： IKv in PASMCs was increased significantly by H2O2 and the increase depended on the concentration in normoxia. Current -voltage relationship curve shifted to the left. CONCLUSION： Hydrogen peroxide is an important K^＋ channel opener.

关 键 词：过氧化氢 平滑肌细胞 钾通道 电压门控 膜片钳术 

分 类 号：R965[医药卫生—药理学]