胰岛素和IGF-1对成骨样细胞MG63 Ⅰ型胶原基因表达的影响  

Effect of insulin and IGF-1 on expression of typeⅠcollagen of human osteosarcoma cell line MG 63

作　　者：马艳芬[1] 李万根[1] 陈澍[1] 

机构地区：[1]广州医学院第二附属医院内分泌科

出　　处：《中国骨质疏松杂志》2006年第5期466-469,共4页Chinese Journal of Osteoporosis

基　　金：广东省自然科学基金资助项目(000333)

摘　　要：目的应用胰岛素(INSU)、胰岛素样生长因子-1(IGF-1)干预体外培养的人成骨样细胞MG63,并以17-雌二醇(E2)做阳性对照。观察药物干预对MG63细胞Ⅰ型胶原(COL1)表达的影响。方法用DMEM培养基培养MG63细胞,并以INSU、IGF-1、E2进行干预。抽提各样本总RNA后,行逆转录并对COL1的cDNA进行荧光扩增和定量。结果各药物组内基因拷贝数(COPYS)均有统计学差异(P<0·05),各组药效呈浓度依赖性增加趋势。在本实验所选的药物浓度范围内,INSU、IGF-1、E2各药物组的最高药效浓度均为本实验所用的最高药物浓度,分别为2×10-7mol/L、10-7mol/L、10-6mol/L。3组间有统计学差异(P<0·05),INSU和IGF-1组COPYS增长率明显大于E2组,INSU和IGF-1组比较无统计学差异。结论INSU、IGF-1和E2一样,均有显著促进MG63分化的作用。Objective To investigate the effect of insulin （INSU） and insulin like growth factor-Ⅰ （IGF- Ⅰ ） on the expression of type Ⅰ collagen（COL1 ） with positive of 17-β estradiol（E2 ） and to explore the pathogenesis of the osteoporosis. Methods MG 63 cells（ 105/mL） were cultured in the DMEM medium and exposed to INSU, IGF-1 or E2 for 48 hours with 0.1% bovine serum albumin phenol-free DMEM. The total RNA of each sample was extracted and reverse transcriptions was performed to get the eDNA chains of COL1. Fluorescence real time quantitative PCR assay was carried out to examine the mRNA expression of COL1. The nest primers were used in the PCR assay. ANOVA and post hoe analysis were performed in SPSS 11.0 software. Results The expression of mRNA for COL1 was significantly different among the three groups （ P 〈 0.05） and the effect of each medication increased dose-dependently. The most significant effect was observed at the highest concentration of each medication applied in this study （INSU 2 × 10^-7 mol/L, IGF-1 10-7 mol/L and 17-[3 estradiol 10-6 mol/L）. The increase rates of COL1 mRNA in INSU, IGF-1 group was significantly higher than that of E2 group （ P 〈 0.05）, but not between the groups of INSU and IGF-1 （ P 〈 0.05 ）. Conclusions INSU and IGF-1 can improved the differentiation of MG 63 as E2.

关 键 词：胰岛素 IGF-1 MG 63细胞 分化 

分 类 号：R587.2[医药卫生—内分泌]