生后早期听觉剥夺、经验改变大鼠听皮层NMDA受体NR2B蛋白表达  

Early Auditory Deprivation and Experience Modify The Expression of NMDA Receptor Subunit NR2B Protein in Rat Auditory Cortex

作　　者：吴秀梅[1] 张凌[1] 许兢宏[1] 徐凤[1] 杨文伟[1] 张季平[1] 孙心德[1] 

机构地区：[1]华东师范大学生命科学学院,脑科学研究中心,上海200062

出　　处：《生物化学与生物物理进展》2006年第11期1080-1085,共6页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目(30170313;90208012;30570595);上海市启明星计划项目(05QMX1420);上海市曙光计划项目(05SG28).~~

摘　　要：应用蛋白质印迹检测技术,研究早期听觉剥夺、经验对大鼠听皮层NMDA受体NR2B蛋白表达的影响.结果表明,听觉剥夺使生后14天龄组和28天龄组动物听皮层NR2B蛋白表达水平明显下降(P<0.05,P<0.01),听觉剥夺7天后再给予纯音暴露则又使NR2B表达水平明显提高(P<0.05),呈现双向调节趋势.听觉剥夺和纯音暴露对生后42天龄组大鼠听皮层NR2B表达不再产生明显调节作用(P>0.05).结果提示,在大鼠生后发育关键期,听觉剥夺、经验可改变听皮层NMDA受体NR2B蛋白表达水平.研究结果为研究感觉功能发育可塑性的机制提供了重要实验资料.Using Western blotting technique, the effects of auditory deprivation and tone exposure on the relative expression level （REL） of NMDA receptor subunit NR2B protein of rat auditory cortex during early postnatal days were studied. The results showed that the NR2B protein expression of PND14 and PND28 decreased significantly （P 〈 0.05, P 〈 0.01） when auditory deprivation for 7 days. Tone exposure after auditory deprivation for 7 days made NR2B protein level increased significantly （P 〈 0.05）. It showed bidirectional regulation of NMDA receptor subunit NB2B protein. No significant effects of auditory deprivation and tone exposure on the relative expression level of NR2B protein of PND42 （P 〉 0.05） were found. The results indicated that, auditory deprivation and experience can modify the protein expression level of NMDA receptor subunit NR2B during the critical period of rat posmatal development. The findings provided important data to the study of the mechanisms for the developmental plasticity of sensory functions.

关 键 词：大鼠 听皮层 NR2B 听觉剥夺 听觉经验 蛋白质印迹 

分 类 号：Q6[生物学—生物物理学]