热暴露大鼠心肌细胞钙稳态的变化  

THE CHANGE OF INTRACELLULAR CALCIUM HOMEOSTASIS IN HEAT-EXPOSED CARDIOMYOCYTES

作　　者：杨晓昊[1] 李文选[1] 程素琦[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所

出　　处：《解放军预防医学杂志》1996年第4期261-264,共4页Journal of Preventive Medicine of Chinese People's Liberation Army

摘　　要：本文观察了急慢性热暴露后培养乳鼠心肌细胞钙内流、钙调素（ＣａＭ）及Ｃａ￣（２＋）－ＡＴＰ酶活性的变化。结果表明：热暴露后Ｃａ￣（２＋）跨心肌细胞肌膜内流增加，细胞匀浆Ｃａ￣（２＋）－ＡＴＰ酶活性具有下降趋势，活性ＣａＭ含量下降，致心肌细胞内游离钙浓度升高。提示：热暴露后心肌细胞钙稳态发生改变，并可能是心肌细胞损伤的重要原因之一。慢性热暴露后心肌细胞对热损害的反应性有所降低、适应性有所增强。he￣(45) Ca influx,active calmodulin content and activity of Ca￣(2+) A TPase from cuIturedcardiomyocytes of neonatal rats after exposure to 37℃,43℃ 40 minutes for 1 time and 10times respectively in vitro were measured.The results showed that :(1)the activity of Ca￣(2+)-ATPase had a tendency to decrease after increase of the exposed temperature;(2)after ex-posed to 43 ℃,the mean fluorescent intensity of cardiomyocytes, which represents relativelyactive calmodulin content,was decreased;(3)the￣(45) Ca uptake in 5 minutes and 120 minutes at43℃ increased significantly, as compared with that at 37℃,It is suggested that the increased Ca￣(2+) inflow and decreased content of active calmodulin lead to the increase of intracellularfree Ca￣(2+) concentration,i.e., the change of intracellular rcalcium homeostasis,which may beone of the main causes of cardiac functional disorder in heat exposure.

关 键 词：热暴露 心肌细胞 钙稳态 

分 类 号：R594.1[医药卫生—内科学]