氟烷基因在三江白猪猪群、迪卡配套系猪B系、亚洲野猪中的分布及其对肉质的影响  

Distributing probability of three genotypes of RYR1 of Sanjiang White pigs

作　　者：耿忠诚[1] 袁子国[1] 刘德贵[2] 靳锐[1] 陈建华[1] 刘丽丽[1] 迟兆江[3] 

机构地区：[1]黑龙江八一农垦大学 动物科技学院,黑龙江大庆163319 [2]北京养猪育种中心,北京100085 [3]黑龙江省农垦总局 科技局,黑龙江哈尔滨150020

出　　处：《黑龙江畜牧兽医》2006年第11期19-22,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：黑龙江省科技攻关项目(GB01B105-01)

摘　　要：试验随机选取138头迪卡配套系猪B系、216头三江白猪和192头亚洲野猪提取基因组DNA进行聚合酶链式反应,得到RYR_1基因特异片段,经过双酶切鉴定,测得Hal^N基因频率分别为87．31%、92．13%、92．97%；Hal^n的基因频率分别为12．69%、7．87%、7．03%。然后对3种氟烷基因型的猪进行肉质鉴定。138 DIKERS,216 white pigs, 198 Sus scrofa were selected randomly, selecting blood samples from Sanjang White pigs,the DNA were distilled in blood and purified , by PCR amplified and enzyme digestied , the genotypes of RYR1 was analysised. The results indicated that ： after conditioning optimized by PCR , a fragment of 659 bp was amplified by PCR , the fragment was digested by Hha Ⅰ and HgiA Ⅰ , three genotypes of Hal^N Hal^N , Hal^N Hal^n, Hal^n Hal^n were gained . The frequency of HalN genotype among three species was 87.31% ,92.13% ,92.97% in turn . The frequency of Haln genotype was 12.69%, 7.87% ,7.03%.

关 键 词：三江白猪 聚合酶链反应 氟烷基因 肉质 

分 类 号：S814.8[农业科学—畜牧学]