趋化因子SDF-1及其受体对卵巢癌细胞增殖及迁移的影响  

Effects of chemokine stromal cell- derived factor-1 and its receptor CXCR4 on the proliferation and migration of ovarian cancer cells

作　　者：林晓琰[1] 王泽华[1] 王晓翊[1] 汪宏波[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院妇产科,武汉市430022

出　　处：《实用医学杂志》2006年第22期2580-2582,共3页The Journal of Practical Medicine

基　　金：湖北省卫生厅科研基金资助项目(编号:JX1A07)

摘　　要：目的:探讨趋化因子受体CXCR4在人卵巢癌细胞中的表达及SDF-1/CXCR4系统与卵巢癌细胞恶性表现的关系。方法:采用RT-PCR检测卵巢癌细胞系SW626中CXCR4的表达,MTT法检测SW626细胞增殖能力的变化,通过体外微孔隔离室板(Transwell)检测SDF-1对SW626细胞迁移及CXCR4的封闭对其迁移的影响。结果:CXCR4mRNA在SW626细胞呈阳性表达,抗CXCR4单克隆抗体(20μg/mL)对SW626细胞增殖有明显的抑制作用(P<0.05),SDF-1可促进SW626细胞的迁移,CXCR4的封闭能抑制SDF-1对SW626迁移的这种促进作用(P<0.05)。结论:SDF-1及其受体CXCR4的相互作用可能在卵巢癌细胞增殖和迁移中发挥着重要作用,干扰SDF-1/CXCR4的相互作用可能成为治疗卵巢癌的一个有意义的靶点和策略。Objective To investigate the expression of CXCR4 in human ovarian cancer cells and the relationship between SDF-1/CXCR4 system and the malignity of ovarian cancer ceils, Methods Expression of CXCR4 mRNA in ovarian cancer cell llne SW626 was detected by reverse transcriptase-polymerase chain reaction （RT-PCR）; the proliferation of SW626 cells was identified by MTT method; Transweil was used to analyze the migration of SW626 cells in presence of SDF-1 or when CXCR4 was blocked by anti-CXCR4 McAh. Results CXCR4 mRNA was expressed in SW626 ceils; anti-CXCR4 McAh （20 μg/mL） inhibited the proliferation of SW626 cells（ P 〈 0. 05）; SDF-1 induced the migration of SW626 cells, which could he effectively blocked by anti-CXCR4 McAh（ P 〈 0.05）, Conclusion SDF-1 and CXCR4 may play an important role in the proliferation and migration of ovarian cancer cells, and interfering the interaction of SDF-1 and CXCR4 can he a meaningful therapeutic target and strategy.

关 键 词：卵巢肿瘤 趋化因子类 受体 SDF-1 CXCR4 

分 类 号：R737.31[医药卫生—肿瘤]