不同基因型新城疫病毒强毒株对鸡的致病性  

Pathogenicity of Different Genotypes of Velogenic Newcastle Disease Virus to Chickens

作　　者：陈立功[1] 万洪全[2] 郝延刚[1] 袁万哲[1] 吴力力[2] 宋红芹[2] 陈露[2] 王宝安[2] 

机构地区：[1]河北农业大学动物科技学院,河北保定071001 [2]扬州大学兽医学院,江苏扬州225009

出　　处：《中国兽医学报》2006年第6期594-596,共3页Chinese Journal of Veterinary Science

基　　金：江苏省自然科学基金青年科技创新人才项目(BK2003403);江苏省高校自然科学研究计划项目(03KJB230158);扬州大学高层次人才科研启动基金项目

摘　　要：就不同基因型新城疫病毒强毒株对鸡的致病性进行了比较。用3种基因型(g、d亚型、型)新城疫病毒强毒株分别人工感染25日龄雏鸡,结果各株病毒接种鸡均100%发病、100%死亡,表现出嗜内脏型新城疫病毒感染引起的典型的新城疫症状和病理变化。免疫组化检测发现,攻毒鸡多种组织器官中能检测到病毒抗原,在能检测到病毒抗原的器官中,病毒抗原主要定位于淋巴细胞、网状细胞、巨噬细胞、肝细胞及各种上皮细胞的胞浆内;新城疫病毒对肠相关淋巴组织的亲嗜性优先于附近的上皮。本试验结果表明,基因g、d亚型、型新城疫病毒强毒株对鸡均具有高度致病性。The present study investigated the pathogenicity of velogenic ND viruses of 3 different genotypes （Ⅵ g, Ⅶd and Ⅸ ） in 25-day-old chickens. As a result, both the morbidity and mortality of the challenged chickens was 100%, while the control birds remained healthy. Chickens showed clinical signs and pathological alternations similar to those of chickens infected with viscerotropic strains of NDV. The localization and distribution of the viral antigen in tissues from the infected chickens were investigated by using immunohistochemical （IHC） staining for the detection of NDV F protein. The viral antigen could be detected frequently in 20 organs and tissues. Specific staining was localized in the cytoplasma of lymphocytes, reticulocytes, maerophages, hepatocytes as well as various epithelial cells. There was marked preferential viral tropism for the lymphoid aggregates associated with the gastrointestinal （GI） tract rather than for nearby epithelial cells. Pathologic findings in association with IHC staining showed that the velogenic ND viruses of the 3 genotypes caused systemic illness involving many organs and tissues in chickens.

关 键 词：新城疫病毒 鸡 基因型 免疫组化 致病性 

分 类 号：S852.65[农业科学—基础兽医学]