3株鹅源新城疫病毒的分子特征和遗传发生分析  

Molecular Characterization and Phylogenetic Tree of Three Isolates of Newcastle Disease Virus from Geese

作　　者：焦库华[1] 马晓丹[1] 马建云[1] 刘文博[1] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009

出　　处：《中国兽医学报》2006年第6期600-602,共3页Chinese Journal of Veterinary Science

基　　金：扬州大学自然科学基金项目(VK0413169)

摘　　要：2005年分离的3株鹅源新城疫病毒毒株经毒力（MDT）检测为强毒株，鸡胚平均死亡时间为48～60h。RT—PCR扩增出了融合蛋白（F）基因，测定相应的核苷酸序列并进行遗传发生分析和酶切位点分析。对基因编码的氨基酸序列进行了推导，3个试验毒株的蛋白酶裂解激活位点附近的氨基酸序列均为112R（K）RQKR↓F117，为强毒特征序列。根据绘制的系统进化发生树和F基因上3种限制性内切酶（RE）位点分布．确定了这3个毒株均为基因Ⅶ型。以上证据表明该基因型的新城疫病毒在我国鹅群的流行占据一定优势。Three Newcastle disease virus isolates from geese in 2005 in China were characterized. All of them were virulent strains based on the MDT（mean death dime）. The genes of fusion protein were sequenced. The amino acid sequence between 112 to 117 were 112R（K）RQKR ↓ FllT. The restriction enzyme cleavage site mapping of the fusion protein gene were between nucleotides 334 and 1 682 and by sequence analysis between nucleotides 47 and 420. Both methods revealed these isolates belong to genotype Ⅶc. The strains of this genotype should be considered as responsible for the outbreaks in geese and chickens in recent years.

关 键 词：鹅 新城疫病毒 遗传发生树 

分 类 号：S852.65[农业科学—基础兽医学]