转化型人参皂甙的生物活性:体外实验研究  

The Bioactivities of Transformed Ginsenosides: An in vitro Experiment Study

作　　者：许小琴 金文杰 李金贵 付本懂[2] 王鲁[2] 楮秀玲[2] 郭志廷[2] 韦旭斌[2] 

机构地区：[1]扬州大学,江苏扬州225009 [2]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国兽医学报》2006年第6期653-656,共4页Chinese Journal of Veterinary Science

基　　金：国家自然基金资助项目(30471272)

摘　　要：将人参总皂甙经化学转化成转化型人参皂甙后分离得8个组分，在体外细胞上进行了转化型人参皂甙8个组分对CEF细胞毒性、生长活性、诱导肿瘤细胞凋亡、诱导单克隆抗体生成以及抗IBDV、AIV（H9）、NDV、MDV病毒的活性研究。结果发现，转化型人参皂甙各组分的细胞毒性作用在23．26～39．53mg／L之间；对鸡胚成纤维细胞（CEF）的生长和活性呈促进作用的工作浓度在10～20mg／L之间；转化型人参皂甙8号组分12．5mg／L诱导48h后，肝癌细胞7402株有60％的细胞凋亡，大鼠脑胶质瘤细胞C6株有35％的细胞凋亡；3、4、7号组分12．5mg／L和6．25mg／L对单克隆抗体6E6株杂交瘤细胞和单克隆抗体10B11株杂交瘤细胞的单抗生成具有明显促进作用，其HI效价为2^6～7与对照组的2^5相比有明显差异，IFA效价为10^4～5与对照组的10^2相比有极显著差异；4、7、8号组分15mg／L阻斯IBDV、AIV（H9）、NDV感染CEF细胞的作用都达到了51．1％以上，抑制IBDV、AIV（H9）、NDV在CEF上增殖的作用都在30％以上；6、7号组分12．5mg／L在阻断感染、抑制增殖、直接杀灭3种方式的抗MDV—RB1B强毒株在SPF鸡CEF细胞上的空斑减数率都达了到ACV抗MDV空斑减数率的80％，其中4、6号组分对MDV—RBIB株的直接杀灭作用与ACV的杀灭作用相等。表明人参总皂甙经化学处理转变成转化型人参皂甙，可明显改变其生物学活性。The transformed ginsenosides（TG） were made of ginsenosides by chemical method and the eight bioactive ingredients isolated from TG were used to evaluate their toxicity and growth-enhancing effect to chicken embryo fihrohlast （CEF） cells in vitro. The bioactivities of apoptosis inducing in hepatocellular carcinoma cell line （7042） cells and monoclonal antibod- ies producing in hybridoma CG cell line cells were investigated, and the bioactivities of these eight TG against IBDV, AIV （H9）, NDV and MDV were also examined. The results showed that the cell toxicity of the eight TG ingredients appeared at the dose from 23.26 to 39.53 mg/L. however, they could enhance the growth and activity of CEF cells at the dose from 10 to 20 mg/ L, 60% apoptosis occurring in hepatocellular carcinoma cell 1ine（7042） cells induced by TG-8 at the dose of 12.5 mg/L were found when the cells exposed to TG-8 for 48h and the 35% apoptosis in rat glioma cell line C6 cells with the same conditions. TG-3, TG-4 and TG-7 enhanced obviously the production of monoclonal antibodies in both 6E6 and 10B11 cell lines at the dose of 12.5 mg/L or 6.25 mg/L. The HI triers of the groups treated with TG-3, TG-4 and TG-7 were up to 2^6-2^7, which were obviously different from 25 of the control group, and the IFA titers 10^4-10^5 of the groups exposed to TG-3, TG-4 and TG-7 were significantly higher than 102of the control group. The infection of IBDV, AIV（H9） and NDV in CEF cells could be blocked respectively by TG-4, TG-7 and TG-8 at the dose of 15 mg/L, with a high blocking rate over than 51.1%. The reproduction of IBDV, AIV（H9） and NDV in 30% CEF cells were also inhibited. The infection-blocking, reproduction-inhibiting and directly inactivating role of TG-6 or TG-7 at the dose of 12.5 mg/L against MDV-RB1B were found in CEF cells, and the plaques of MDV in CEF cells reduced by 80% as compared to those of aciclovir control group. The inactivating role of TG-4 or TG-6 to MDV-RB1B cells was equal to that of aciclovir group. This

关 键 词：转化型人参皂甙 细胞毒性 细胞活性 肿瘤细胞凋亡 单克隆抗体 病毒 

分 类 号：S859[农业科学—临床兽医学]