肝素和钙离子载体诱导波尔山羊精子体外获能和体外受精  

Capacitation and Fertilization of Boer Goat Sperm Induced by Heparin or Ionophore A23187(I-A) in vitro

作　　者：马恒东[1] 梁鑫[1] 

机构地区：[1]四川农业大学动物科技学院,四川雅安625014

出　　处：《中国兽医学报》2006年第6期701-703,共3页Chinese Journal of Veterinary Science

基　　金：四川省教育厅重点项目资助(2003A016)

摘　　要：运用肝素、钙离子载体(IA)协同咖啡因诱导波尔山羊精子体外获能,利用去透明带金黄地鼠卵穿透试验检测了精子的获能效果。结果发现:精卵孵育4h和6h时的穿透率均高于2h时的穿透率;肝素和钙离子载体对精子获能有明显的促进作用,与未添加组相比差异极显著(P<0.01);20mg/L和50mg/L的肝素剂量组,去透明带金黄地鼠卵穿透率为64.7%、68.4%;钙离子载体的添加剂量在0.3μmol/L时,穿透率达到68.2%;咖啡因与肝素、钙离子载体具有明显的协同作用。将获能的精子与山羊卵母细胞进行体外受精孵育17h,也获得了60%以上的受精率。The present experiment was carried out were to select a disirable culture system in vitro capacitation and fertilization for Boer goat spermatozoa. After addition of capacited sperms treated with heparin or IA to the hamster eggs,penetration rate of sperms incubated for 4-6 h was significantly higher than that for 2 h. Penetration rate of goat sperms treated with 20 mg/L heparin or 0.3 μmol/L IA was 64.7% or 68.2% respectively. Caffeine had a synergetic effect in combination with heparin or IA on enhancement of penetration rate. The higher fertilization rate was obtained when oocytes and sperm cell were incubated for 17 h than 8 h（P〈0. 01）.

关 键 词：波尔山羊 精子体外获能 肝素 钙离子载体 去透明带金黄地鼠卵 体外受精 

分 类 号：S827[农业科学—畜牧学]