核因子-κB和IL-8在激素敏感型单纯性肾病病毒基因反式激活过程中表达变化及糖皮质激素的影响  

Expression of Nuclear Factor-κB and Interleukin-8 During Viral Transactivation of Transcription in Steriod Responsive Simple Nephrotic Syndrome and the Effect of Glucocortoid.

作　　者：陶于洪[1] 王峥[1] 李缠生[1] 朱小石[1] 

机构地区：[1]四川大学华西第二医院儿科,四川成都610041

出　　处：《四川医学》2006年第11期1101-1103,共3页Sichuan Medical Journal

基　　金：国家自然科学基金项目资助(39870747)

摘　　要：目的研究激素敏感型单纯性肾病(SRSNS)病毒基因反式激活中核因子-κB(NF-κB)活性和IL-8表达,以及糖皮质激素的抑制作用。方法采用电泳迁移率改变实验、RT-PCR和ELISA分别测定SRSNS活动期(包括初发和复发)患儿外周血单个核细胞(PBMCs)NF-κB活性、呼吸道病毒基因、病毒抗体及IL-8浓度。结果SRSNS活动期NF-κB活性和IL-8水平增加;NF-κB活性与呼吸道病毒检出率呈正相关。SRSNS活动期复发组NF-κB活性和IL-8浓度低于初发组。结论NF-κB和IL-8参与SRSNS病毒基因反式激活。糖皮质激素抑制病毒基因反式激活与其对NF-κB和IL-8的调节作用有关。Objective To investigate the activity of NF-κB and the concentration of IL-8 in steroid responsive simple nephrotic syndrome（SRSNS）during viral transactivation of transcription, and to determine glucoeortoid-mediated inhibition effect on the activity of NF-κB and the expression of IL-8. Methods Electro-mobility shift assays, RT-PCR and ELISA were used to detect the activity of NF- -κB, the gene expression of respiratory tract viruses （ including respiratory syncytial virus, influenza virus）in peripheral blood mononuclear cells（PBMCs）and the levels of viral antibody and IL-8 in plasma from children with SRSNS at active stage （including incipient stage and relapse stage）, respectively. Results The activity of NF-κB and the level of IL-8 in SRSNS at active stage were elevated, and there was a positive linear correlation trend between the activity of NF-κB and the gene expression of respiratory tract viruses in SRSNS at active stage .The activity of NF--κB and the level of IL-8 in SRSNS at relapse stage was lower than those in SRSNS at incipient stage. Coneluslon NF-κB and IL-8 may play a key role in the viral transactivation of transcription in SRSNS. Glucoeortoid inhibits viral transactivation of transcription through regulating the activity of NF-κB and the expression of IL-8.

关 键 词：肾病综合征 核因子-ΚB 病毒基因反式激活 呼吸道病毒 糖皮质激素 

分 类 号：R726.91[医药卫生—儿科]