APP17肽对心肺复苏大鼠海马神经元IR IRS-1表达的影响  

Effect of APP17 peptide on the hippocampal neuron expression of IR,IRS-1 in CPR rats

作　　者：王晶[1] 路毅[1] 王蓉[2] 赵妍[1] 盛树力[2] 

机构地区：[1]首都医科大学宣武医院急诊科,北京100053 [2]首都医科大学宣武医院教育部神经变性病学重点实验室

出　　处：《中国急救医学》2006年第11期831-833,I0002,共4页Chinese Journal of Critical Care Medicine

摘　　要：目的观察APP17肽对心肺复苏大鼠海马神经元CA1区胰岛素受体(IR)、胰岛素受体底物-1(IRS-1)表达的影响。方法24只SD大鼠随机分为3组：假手术对照组(对照组)、心肺复苏组(复苏组)、APP17肽组,每组8只。用室息法制作大鼠心肺复苏模型。APP17肽组于自主循环恢复(ROSC)时立即静脉注射APP17肽3．33μg/100 g体质量,约5 mL；对照组和复苏组给予等剂量生理盐水。2 h后断头取脑行免疫组化染色,观察3组大鼠海马CAl区神经元IR、IRS-1表达的变化。结果与对照组相比复苏组大鼠海马CA1区IR、IRS-1染色阳性神经元明显减少(P＜0．05-0．01)差异有统计学意义；与复苏组相比APP17肽组IR、IRS-1阳性神经元明显增加,差异有统计学意义(P＜0．05～0．01)。结论心肺复苏大鼠海马神经元IR、IRS-1表达降低,复苏早期给予APP17肽干预可使其表达恢复正常或接近正常。Objective To observed the effect of APP17 peptide （ β - Amyloid precursor protein,APP,319 -335 peptide section）, on the hippocampal neuron expression of Insulin receptor（ IR ）, Insulin receptor substrate - 1 （ IRS - 1 ） in cardiopulmonary resuscitation （ CPR ） rats. Method The SD rats were divided averagely into three groups： normal control group, CPR group and treated with APP17 peptide group. These rats were used to make CPR models with apnoea method. APP17 peptide 3.33 βg/100 g weight,a- bout 5 mL was injected after return of spontaneous circulation （ ROSC ） immediately in APPI7 group, while the same dose of Sodium Chloride was injected in CPR group. After 2 hours of ROSC, the rats were caused dead and brains were took out. The changes of IR, IRS - 1 in the hippocampal neuronic CAI region were mensurated through immunohistochemistry. Result The expression of IR, IRS - 1 significantly decreased in CPR group as compared with control group （ P 〈0. 05 -0.01 ） , the expression of IR , IRS - 1 significant- ly increased in APPI7 peptide group as compared with CPR group （ P 〈 0. 05 - 0.01 ） . Conclusion The expression of IR , IRS - 1 decreased in the hippocampal neuron in CPR rats, and early administration of neurotrophic factor - APP17 peptide could recover the expression of IR, IRS- 1 to or approximately to the normal level.

关 键 词：心肺复苏:APP17肽 胰岛素受体(IR) 胰岛素受体底物-1(IRS-1) 

分 类 号：R459.7[医药卫生—急诊医学]