金叶败毒颗粒防治豚鼠巨细胞病毒宫内感染  

Study on traditional Chinese medicine Jingyebaidu granules for treatment and prophylaxis of the intrauterine infection with guinea pig cytomegalovirus

作　　者：邓东锐[1] 陈素华[1] 熊锦文[1] 赵婕[1] 乔福元[1] 凌霞珍[1] 闻良珍[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,湖北武汉430030

出　　处：《中国医院药学杂志》2006年第11期1342-1344,共3页Chinese Journal of Hospital Pharmacy

摘　　要：目的：利用豚鼠巨细胞病毒（guinea pig cytomegalovirus．GPCMV）宫内感染模型，探究金叶败毒颗粒防治GPCMV宫内感染的有效性和安全性。方法：①选择无感染史孕早期豚鼠．随机分为3组．感染组（30只）：腹腔接种GPCMV；治疗组（30只）：腹腔接种GPCMV＋灌胃给予中药金叶败毒颗粒；空白对照组（30只）：腹腔灌胃生理盐水。20d后处死豚鼠．采集血液及各脏器组织．检测各组豚鼠的感染情况。②随机选取受孕后昆明小鼠．分为3组．治疗组（30只）：灌胃给予金叶败毒颗粒；阳性对照组（30只）：给予致畸阳性对照药维生素A；空白对照组（30只）：给予温水。追踪母鼠一般情况及胎子生长发育状况。结果：①感染组中93．33％（28／30）出现病毒血症的征象．3．33％（1/30）死亡；治疗组和空白对照组无任何感染征象。感染组和治疗组血GPCMV DNA阳性率分别为100％（30/30）和46．67％（14／30），差异有极显著性（P〈0.01）。②昆明小鼠于器官发生期（孕1～18d）给药并不增加子代生长发育异常的风险，对孕鼠也无明显不良作用。结论：金叶败毒颗粒具有良好的抗GPCMV感染效能，对亲子代均无明显不良反应及致畸效应．因而有望成为孕期防治宫内感染的理想用药。OBJECTIVE To investigate the effect of traditional Chinese medicine -Jinyebaidu granules on the pregnant guinea pigs （GPs） infected with GP cytomegalovirus （GPCMV） and its safety in pregnancy. METHODS ① The pregnant C-Ps in the first trimester were randomly divided into three groups： the infective group was only inoculated with GPCMV intraperioneally; the treatment group was given Jinyehaidu granules through stomach perfusing after being inoculated, and the blank control group was treated with nothing special, The GPs were killed after 20 days and the GPCMV infection rate was examined in the blood and organs, ②The mice from Kunming were randomly divided into three groups ： the treatment group accepted Jinyebaidu granules;the positive control group was treated with vitamin A and the blank control group was given nothing special. The effect on pregnant mice and the growth and development of their offsprings were followed up. RESULTS ① The rates of viremia manifestation and death in the infective group were 93.33 % （28/30） and 3.33% （1/30）, respectively, hut no infection sign was seen in the treatment group and blank control group. GPCMV DNA positive rate of blood in the treatment group was significant lower than that of positive group （P〈0. 01 ）. ② Jinyehaidu granules had no side-effect on the pregnant mice and their offsprings. CONCLUSION Traditional Chinese medicine Jinyebaidu granules markedly reduces the maternal-fetal infective rate of GPCMV. The medicine is safe when used during pregnancy.

关 键 词：金叶败毒颗粒 豚鼠 巨细胞病毒 宫内感染 

分 类 号：R965[医药卫生—药理学]