高效液相色谱法测定氟康唑血药浓度  

The determination of fluconazole concentration in plasma by high-performance liquid chromatography

作　　者：郭美华[1] 张波[2] 杜智敏[2] 马妍妍[1] 

机构地区：[1]哈尔滨医科大学附属第一医院,黑龙江哈尔滨150001 [2]哈尔滨医科大学附属第二医院,黑龙江哈尔滨150086

出　　处：《中国医院药学杂志》2006年第11期1366-1368,共3页Chinese Journal of Hospital Pharmacy

摘　　要：目的:优化氟康唑血药浓度测定方法。方法:采用高效液相色谱(HPLC)法,以非那西丁为内标,Nova-Pak C18柱(150mm×4.6mm,5μm),以0.1mol.L-1磷酸-磷酸二氢钾缓冲液-甲醇(50∶50,v/v,pH7.0)为流动相,在210nm处测定其含量。结果:线性范围为0.312 5~20mg.L-1,提取回收率>86.40%(n=5),方法回收率为92.6%~102.6%,日内RSD≤3.46,日间RSD≤5.71%,且稳定性良好。结论:方法可靠、简便、快速。OBJECTIVE To determine fluconazole concentration in plasma by HPLC.METHODS Fluconazole concentration in plasma was determined by HPLC.Analytical columns:Nova-PakC_(18)(150 mm×(4.6) mm,5 μm);mobile phase:(0.1) mol·L^(-1)H_(3)PO_(4)-KDP buffer(50∶50,v/v,pH(7.0));detection wavelength: 210 nm.RESULTS A linear range was(0.3125)-20 mg·L^(-1).Intra-day and inter-day precisions were less than(3.46%)(n=5) and(5.71%)(n=5),respectively.The extraction recoveries were more than(86.4%)(n=5),and the method recoveries were(92.6%)-(102.6%).CONCLUSION The method is reliable,convenient and more efficient.

关 键 词：氟康唑 血药浓度 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]