高效液相色谱法测定乳腺小叶增生颗粒中芍药苷的含量  

Determination of paeoniflorin in Ruxianxiaoye Zengsheng granules by HPLC

作　　者：童荣生[1] 孙世明[1] 吴正中[1] 

机构地区：[1]四川省医学科学院四川省人民医院,成都四川610072

出　　处：《中国医院药学杂志》2006年第11期1370-1372,共3页Chinese Journal of Hospital Pharmacy

摘　　要：目的：建立乳腺小叶增生颗粒中芍药苷的含量测定方法。以控制该产品的质量。方法：采用HPLC法．色谱柱为Gemini C18（250mm×4．6mm．5μm），柱温：30℃；流动相为乙腈-0．1％磷酸溶液（20：80．v／v）．流速1．0mL·min；检测波长230nm。结果：芍药苷的进样量在0．10024～1．0024μg范围内．与其峰面积有良好的线性关系（r=0．9999），平均加样回收率为100．79％．RSD为1．92％。结论：方法简便易行、准确可靠，可用于乳腺小叶增生颗粒剂的质量控制。OBJECTIVE To establish an HPLC method for determinations of the content of Paeoniflorin in Ruxianxiaoyezengsheng granules. METHODS The assay was performed on a Gemini C18 column （250 mm× 4. 6 mm.5μm） by UV detection at 230nm with acetonitrile-0. 1% phosphoric acid （20：80）as the mobile phase at the flow rate of 1. 0 mL·min ^-1. RESULTS The amount of Paeoniflorin was linear with its area over the range from 0. 1002 to 1. 0024 μg（r= 0. 9999）. The average recovery and the relative standard deviation of Paeoniflorin were 100. 79% and 1.92% （n = 6）.respectively. CONCLUSION The method is be sensitive, accurate and can be used for the quality control of the TCM preparation Ruxianxiaoye Zengsheng granules.

关 键 词：芍药苷 乳腺小叶增生颗粒 HPLC 含量测定 

分 类 号：R927.2[医药卫生—药学]