农杆菌介导的TaLEA_1基因对拟南芥的遗传转化  

Agrobacterium-mediated Genetic Transformation of TaLEA_1 into Arabidopsis thaliana

作　　者：包宇[1] 赵咏梅[2] 俞嘉宁[1] 杨建雄[1] 

机构地区：[1]陕西师范大学生命科学学院,陕西西安710062 [2]西安文理学院生命科学系,陕西西安710065

出　　处：《西安文理学院学报（自然科学版）》2006年第4期31-33,共3页Journal of Xi’an University(Natural Science Edition)

基　　金：国家转基因植物研究与产业化开发专项(J2002-B-001)

摘　　要：将含有TaLEA1基因的表达载体pBI121-TaLEA1经根癌农杆菌介导,利用花粉管通道法转入拟南芥,得T0代种子,抗Kan筛选得转基因植株.经PCR检测,初步证明外源TaLEA1基因已整合到拟南芥基因组中.Mediated by Agrobacterium tumefaciens, expression vector pBI121- TaLEA1 containing TaLEA1 gene was transformed into Arabidopsis thaliana by pollen-tube pathway to obtain seeds of T0-the generation and transgenic plants by Kan resistance screening. It is proved by PCR that TaLEA1 has been integrated into genome of Arabidopsis thaliana.

关 键 词：根癌农杆菌 TaLEA1基因 花粉管通道法 

分 类 号：Q343.1[生物学—遗传学]