心肌肌凝蛋白轻链放射免疫分析及临床初步应用  

Radioimmunoassay of cardiac myosin light chains and its primary clinical application

作　　者：林国城[1] 尚玉琨[1] 陈敏[1] 刘长滨[1] 侯英萍[1] 张裕民[1] 乔宏庆[1] 

机构地区：[1]第四军医大学西京医院核医学科,西安710032

出　　处：《中华核医学杂志》1996年第4期224-226,共3页Chinese Journal of Nuclear Medicine

摘　　要：为提供急性心肌梗塞(AMI)早期血清学诊断法,用改进后的 Sch βler 法提取狗肌凝蛋白轻链(CM-LC),免疫兔获得抗血清。氯胺 T 法标记抗原,建立血清 CM-LC RIA。结果:该方法标准曲线测量范围1～100μg/L,批内、批间 CV 分别是3.2%和9.1%,平均回收率95.3%。正常人30例血清 CM-LC 为3.5±1.5μg/L,20例 AMI 患者血清 CM-LC 为20.2±8.6μg/L,两组间差异非常显著。提示血清 CM-LC RIA 可用于 AMI 早期诊断。PURPOSE To establish a radioimmunoassay (RIA) of cardiac myosin light chains (CM-LC) for early diagnosis of acute myocardial infarction (AMI).METHODS The method established by Sch(?)βbler was modified to purify CM-LC from fresh dog heart.Antiserum was obtained by immunizing rabbits.CM-LC was iodinated by chloramine-T method.Conventional RIA procedure was used.RESULTS Sensitivity of the RIA system was 1μg/L.The intra-assay CV and inter-assay CV were 3.2% and 9.1% respectively,and the mean recovery rate was 95.3%.The serum CM-LC level in 30 normal subjects was 3.5±1.5μg/L, while in 20 patients with AMI was significantly increased (20.2±8.6/μg/L,P<0.01).Increased CL-MC concentration in pa- tients was seen at 5 to 72 hours after the attack of AMI.CONCLUSIONS These results suggest that RIA of CM-LC could be valu- able in the diagnosis of AMI.

关 键 词：肌球蛋白 放射免疫分析 心肌梗塞 血清学诊断 

分 类 号：R542.220.4[医药卫生—心血管疾病]