土壤和植物中乙二胺二琥珀酸的反相液相色谱法测定  

Determination of Ethylenediaminedisuccinic Acid in Soil and Plant Using Reversed Phase High Performance Liquid Chromatography

作　　者：孙小峰[1] 吴龙华[1] 骆永明[1] 

机构地区：[1]中国科学院南京土壤研究所土壤与环境生物修复研究中心

出　　处：《分析化学》2006年第10期1375-1378,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20477048;40125005);国家自然科学基础研究发展规划(973)项目(No.2002CB410809;2005CB121104)资助

摘　　要：乙二胺二琥珀酸（EDDS）可应用于重金属污染土壤的强化植物修复或是土壤淋洗修复.但是现有的检测方法或过于复杂,或检出限太高.本研究建立了应用超声波萃取、硫酸铜衍生化和液相色谱,紫外检测器测定土壤、植物中EDDS含量的方法.其回收率为93%～ 118%,灵敏度较高;检出限达1.5 μmol/L;重现性好,不受其它多种共存离子的干扰.Ethylenediaminedisuccinic acid （EDDS） can be used in chelate enhanced phytoremediation or leaching remediation for heavy metal-polluted soil. But current method have a very high detection limit or complex process. This study describes the development of a method for determining EDDS in soil and plant at low concentration s by high performance liquid. After short sample pretreatment, the samples extracted by supersonic and derived with copper sulfate were analyzed by reversed phase liquid chromatography. The mobile phase is 0.03 mol/L acetate-acetic acid buffer of pH 4.0 with counter ion. Ultraviolet absorbance detection was applied at 254 nm. The method： is simple and a low limit of detection （ 1.5 μmol/L）. The correlation coefficient exceeded 0.99 and the recovery efficiency is between 93% - 118%. It is reliable and the interference from other metal ions was eliminated.

关 键 词：乙二胺二琥珀酸 衍生化 液相色谱法 

分 类 号：O657.72[理学—分析化学]