小尾寒羊精液保存技术研究  

Study on Preservation of Semen in Little-tail Sheep

作　　者：徐振军[1] 闫长亮[1] 郭成[2] 路永强[3] 朱士恩[1] 

机构地区：[1]中国农业大学动物科技学院,北京100094 [2]赤峰学院生命科学系,内蒙古赤峰024000 [3]北京市畜牧兽医站,北京100107

出　　处：《中国畜牧杂志》2006年第21期9-11,共3页Chinese Journal of Animal Science

基　　金：北京市农委试验示范项目

摘　　要：为了充分发挥种公羊的生产潜力,快速扩繁优良群体,本实验以取材方便的小尾寒羊为实验动物,对其精液采用室温、低温和细管法冷冻3种保存方法进行了研究。结果表明:在低温(4±0.5)℃条件下稀释保存小尾寒羊精子,显著优于室温(23±2)℃条件下的保存效果。其中采用II液低温保存绵羊精子的时间和活率(168 h,0.35)显著优于III液(72 h,0.30)I、V液(48 h,0.35)和I液(18 h,0.42)。而采用III液冷冻保存绵羊精液,其解冻后活率(0.532±0.004)极显著高于IV液(0.470±0.003)I、液(0.445±0.004)和II液(0.432±0.012)3种冷冻稀释液(P<0.01)。4种冷冻稀释液冷冻解冻后的精子,均能在室温下6 h内保持0.35以上的活率。To fully bring out the productive potentialities and rapidly expand the fine flock of little-tail sheep, three kinds of techniques on preserving ram semen at room temperature, low temperature and super-low temperature were investigated. The results show that the very good preserving result was achieved at low temperature （4 ＋0,5） ℃ than that at room temperature （23 ± 2） ℃. Among the four kind of solutions, the preserving result of solution Ⅱ（168 h, 0.35） is better than those of Ⅲ（72 h, 0.3）,Ⅳ （48h, 0.35）and Ⅰ（ 18 h, 0.42）at low temperature. However, solution Ⅲ（0.532 ±0.004）is better for cryopreservation of ram semen than those of Ⅳ（0.470± 0.003）,Ⅰ（0.445 ±0.004）and Ⅱ（0.432± 0.012）（P 〈 0.01）.The motilities of four kinds of frozen-thawing sperm are over 0.35 within 6 hours at room temperature.

关 键 词：畜牧学 精液 低温保存 冷冻保存 小尾寒羊 

分 类 号：S827.3[农业科学—畜牧学]