基于自组装单分子层技术的压电石英晶体DNA传感器固定方法的研究  

Immobilization Method on Piezoelectric Quartz Crystal DNA Sensors Based on Monolayer SAM

作　　者：夏涵[1] 府伟灵[1] 陈鸣[1] 王丰[1] 赵渝徽[1] 

机构地区：[1]第三军医大学西南医院,重庆400038

出　　处：《中华医院感染学杂志》2006年第11期1218-1220,1224,共4页Chinese Journal of Nosocomiology

摘　　要：目的探讨一种高效的压电石英晶体DNA传感器固定的方法。方法分别采用常规自组装法(未还原探针法)与探针预先经过还原的自组装法(还原探针法),将针对铜绿假单胞菌的探针固定在DNA传感器的金膜表面,比较不同探针浓度下,探针固定和杂交反应所引起的频率的变化以及所用的时间。结果与未还原探针法相比,还原探针法具有探针固定量大、反应速度快、频率下降明显等优点。结论还原探针法提高了DNA探针的固定效率,优于未还原探针法。OBJECTIVE To discuss a highly effective method to immobilize probe on the surfaces of piezoelectric DNA sensors. METHODS Pseudomonas aeruginosa probe was immobilized on the gold surface of gene sensor array with routine self-assembly method （SAM） （non-reduction method） and SAM with deoxidized probe （reduction method）, respectively. The changes in frequency and time-cost were compared in reactions with different concentrations of probe. RESULTS Reduction method had the advantage of more probe immobilization;less time consumed in testing and higher changes in frequency during the reaction than non-reduction method. CONCLUSIONS Reduction method has a better ability to immobilize probe on the surfaces of piezoelectric DNA sensors.

关 键 词：石英晶体 探针 自组装 铜绿假单胞菌 

分 类 号：R446[医药卫生—诊断学]