抗真菌药敏试验在3种不同琼脂平板的比较  

Antifungal Susceptibility Testing with Three Different Agars:A Comparative Study

作　　者：陈翠敏[1] 府伟灵[1] 张晓兵[1] 龚雅丽[1] 

机构地区：[1]第三军医大学西南医院,重庆400038

出　　处：《中华医院感染学杂志》2006年第11期1307-1309,共3页Chinese Journal of Nosocomiology

摘　　要：目的通过对临床常用的5种抗真菌药物的纸片扩散法药敏试验,对3种真菌药敏培养基进行比较分析。方法选用5种Rosco公司的Neo-Sensitab抗真菌药敏纸片,在Shadomy改良琼脂、RPMI 1640琼脂(RPMI1640)和葡萄糖亚甲蓝M-H琼脂(GMB M-H)3种培养基上对80株临床分离酵母菌检测,以Shadomy改良琼脂为对照。结果对于5种抗真菌药物其敏感性,RPMI 1640琼脂与Shadomy改良琼脂相比较,它们的符合率相同都较高;亚甲蓝MH无其他两种的符合率高,RPMI 1640和GMB M-H与Shadomy相比都显示了较好的相关性(P<0.01)。结论RPMI 1640琼脂可以用于Rosco抗真菌药敏纸片试验,既经济、简便、快速、准确,又有良好的实用性,可以代替Shadomy培养基;MH亚甲蓝培养基对于5-氟胞嘧啶的判读结果与NCCLS相差甚远,对于其他药敏纸片的结果与NCCLS的符合率,也没有另外两种琼脂上做出结果的符合率高,不建议使用M-H(亚甲蓝)琼脂进行Rosco纸片扩散实验。OBJECTIVE To analyze three methods with five antifungals susceptibility test. METHODS Eighty pathogenic yeasts isolated from various specimens were detected by dish diffusion method for yeast susceptibility testing in three different agars [Shadomy＇s modified agar, RPMI 1640 agar, M-H（GMB ）agar]with comparison of the Shadomy＇s modified agar. RESULTS According to sensibility of five antifungals, RPMI 1640 was the same with Shadomy, but GMB M-H was lower with others. Three different agars had more correlability （P〈,0.01）. CONCLUSIONS With the agreements for Neo-Sensitab dish diffusion method for yeast susceptibility testing in RPMI, RPMI 1640 agar is simple to operate. The result is clear to evaluate than other two agars. Its low price and usefulness make it possible to be applied in routine clinical laboratories.

关 键 词：抗真菌药 药敏试验 

分 类 号：R379[医药卫生—病原生物学]