高效液相色谱法测定家兔血浆中Z－47浓度及其在药代动力学研究中的应用  

HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF Z-47 IN KABBIT PLASMA AND ITS APPLICATION TO A PRELIMINARY PHARMACOKINETIC STUDY

作　　者：钟大放[1] 陈仁弟[1] 李雪庆[1] 秦文杰[1] 顾景凯[1] 

机构地区：[1]沈阳药科大学药学系

出　　处：《沈阳药科大学学报》1996年第3期201-206,共6页Journal of Shenyang Pharmaceutical University

摘　　要：本文建立了测定家兔血浆中Ｚ－４７（３Ｈ－１，２－二氢－２－（４－甲基苯胺基）甲基－１－吡咯里嗪酮）的高效液相色谱法。选用μ－ＢｏｎｄａｐａｋＣ１８色谱柱，流动相为甲醇－磷酸二氢铵缓冲液（ｐＨ＝４）（３：２，Ｖ／Ｖ）。以安定为内标物。血浆样品先经碱化，用乙醚－正己烷（１：１，Ｖ／Ｖ）提取，分离有机相，再以１０％磷酸萃取，水相２０μＬ进样，紫外检测波长２９０ｎｍ。本法专属性强，精密度、准确性符合有关要求，操作简便。对６只家兔口服给药后应用本法成功地进行了初步的药代动力学研究。A high-performance liquid chromatographic method for the quantitative determination of Z-47(3H-1,2-dihydro-2-(4-methylphenylamino)methtl-1-pyrrolizinone),a new antiinflammatory and analgesic agent,in rabbit plasma is presented.The method uses a μ-Bondapak C18 column and a mobile phase containing methanol and ammonium dihydrogen phosphate buffer(pH4)(3:2),with diazepam as the internal standard.The analyte is detected by UV at 290 nm.Calibration curves are linear over the range of 30-3000 μg/L.The limit of quantitation is 30 ng/mL for Z-47 in the plasma.The method proves to be simple and sensitive with good specificity,precision and accuracy.It has been successfully applied to a preliminary study of Z-47 pharmacokinetics involving six rabbits.The plasma samples have been alkalified and then extracted with ether-n-hexane(1:1).The organic phase has been separated and extracted with 10% phosphoric acid subsequently.20 μL of the aqueous phase has been injected into the chromatographic system.

关 键 词：甲基苯胺基 吡咯里嗪酮 高效液相色谱 血药浓度 

分 类 号：R969.1[医药卫生—药理学] R971.1[医药卫生—药学]