MDS患者外周血中TCRVβ_(1-24)-Dβ1sjTRECs的分析  

Analysis of TCR Vβ_(1-24)-Dβ1 sjTRECs in peripheral blood from patients with myelodysplastic syndrome

作　　者：胡刚[1] 李扬秋[1] 尹青松[1] 陈少华[1] 杨力建[1] 李闯[1] 杜欣[2] 

机构地区：[1]暨南大学医学院血液病研究所,广州510632 [2]广东省人民医院血液科,广州510080

出　　处：《免疫学杂志》2006年第6期656-658,663,共4页Immunological Journal

基　　金：国家自然科学基金(30270579);广东省自然科学基金重点项目(23001);广东省教育厅"千百十工程"优秀人才培养基金(Q校02022);国务院侨办重点学科基金资助

摘　　要：目的检测骨髓增生异常综合征(MDS)患者外周血中TCRVβ1-24-Dβ1sjTRECs的存在情况。方法利用半巢式PCR扩增9例MDS患者外周血单个核细胞DNA中TCRβ链基因重排时形成的T细胞受体DNA删除环(Vβ1-24-Dβ1sjTRECs),10例正常人外周血作为对照。结果所有TCRVβ1-24-Dβ1sjTRECs在正常人组外周血T细胞DNA中均有检出,除TCRVβ12-Dβ1sjTRECs和TCRVβ19-Dβ1sjTRECs外,其余TCRVβ-Dβ1sjTRECs在MDS患者外周血T细胞DNA中均可检。大多数TCRVβ1-24-Dβ1sjTRECs在正常人组中的检出率较MDS患者高,但其中只有6种TCRVβ-Dβ1sjTRECs(分别是TCRVβ3、12、13、15、21、22-Dβ1sjTRECs)的检出率差异有统计学意义。结论本研究率先提供了MDS患者外周血T细胞中TCRVβ-Dβ1sjTRECs的存在情况。MDS患者TCRVβ-Dβ1sjTRECs出现频率低于正常人提示其胸腺近期输出功能存在部分缺陷情况。Objective To detect the existence of TCR Vβ1-24-Dβ1 sjTRECs in peripheral blood from patients with myelodysplastic syndrome （MDS）. Methods The TCR Vβ1-24Dβ1 sjTRECs were amplified in genomic DNA of peripheral blood mononuclear cells （PBMC） from 9 patients with MDS by using semi-nested PCR, with 10 normal individuals served as control. Results All of the TCR Vβ1-24-Dβ1 sjTRECs were detected in normal individuals. Except TCR Vβ12-Dβ1 sjTRECs and TCR Vβ19-Dβ1 sjTRECs, all the other TCR Vβ-Dβ1 sjTRECs were detected in MDS patients. The frequency of most TCR Vβ1-24-Dβ1 sjTRECs in normal individuals was higher than those in MDS patients, and 6 out of all （TCR Vβ3, 12, 13, 15, 21, 22-Dβ1 sjTRECs） showed statistical significance. Conclusion This is the first report of the detection of TCR Vβ-Dβ1 sjTRECs in MDS patients. The lower frequency in some of TCR Vβ-Dβ1 sjTRECs in MDS patients suggests that there might be partial defection in recent thymic output function.

关 键 词：MDS NAIVE T细胞 TRECS 胸腺近期输出功能 

分 类 号：R551[医药卫生—血液循环系统疾病]