普通小鼠和裸小鼠呼吸道合胞病毒感染模型比较  

Comparison between respiratory syncytial virus infected models of common mice and nude mice

作　　者：周娟[1] 胡敏[1] 王莉佳[1] 崔玉霞[1] 刘雪梅[1] 杨锡强[1] 

机构地区：[1]重庆医科大学附属儿童医院免疫研究室,重庆400014

出　　处：《重庆医科大学学报》2006年第5期631-635,657,共6页Journal of Chongqing Medical University

基　　金：国家自然科学基金(NO.30340045)

摘　　要：目的:比较研究BALB/c小鼠和裸小鼠呼吸道合胞病毒(RSV)感染模型的特点,建立理想的动物模型。方法:BALB/c鼠和裸鼠感染RSV后不同时间空斑形成实验检测肺组织病毒滴度,计数支气管肺泡灌洗液白细胞总数和分类情况,免疫荧光和免疫组化检测RSV抗原,光镜和电镜检查肺组织病理学改变。结果:感染后裸鼠肺组织RSV滴度明显高于BALB/c鼠,两者都在第3天达峰值,带毒时间分别为大于9天和6天,免疫组化和免疫荧光检测到RSV抗原,支气管肺泡灌洗液和肺组织病理学检查表现的以淋巴细胞浸润为主的炎症反应,裸鼠明显重于BALB/c鼠。结论:裸鼠和BALB/c鼠RSV感染特点相似,感染程度更重,有利于抗RSV药物效果评价,是较理想的RSV感染小鼠模型。Objective：To compare the infection of respiratory syncytial virus in common BALB/c mice and nude BALB/c mice to establish ideal animal model. Methods： At various time after RSV infection in BALB/c mice and nude mice, viral titers of lung tissue were assayed by plaque forming experiment,total leukocytes and differential subgroup cells in bronchoalveolar lavage fluid （ BALF ）were counted.RSV antigens were detected by immunofluorescent assay in BALF and immunohistochemistry in situ of lung tissue, lung histologys were identified by light microscope and electronical microscope assay. Results ： RSV was isolated from lung tissue of BALB/c mice and nude mice.RSV antigen was detected by immunofluorescent assay in BALF and by immunohistochemistry in situ of lung tissue in both mouse, furthermore the mean light density of RSV antigen was higher in nude mice （ 0.277±0.085 ）than in BALB/c mice （ 0.128 ±0.031 ）. The viral titers in both mouse peaked at the 3rd day post infection, and higher viral titer in nude mice than BALB/c mice.The viral titers detecting duration in lung tissue of BALB/c mice and nude mice were respectively 6 days and over 9 days after infection.The total leukocyte number in BALF significantly increased in infected BALB/c mice and nude mice compared with the control mice, and the peaks were the 6th day in BALB/c mice and the 9th day in nude mice.The leukocyte subgroups in turn were lymphocyte, maerophage, neutrophil and eosinophil. Interstitial inflammations with vast lymphocytes infiltrating were identified in lung histology of both mouse. On the 6th day, the more severe inflammation was detected than on the 3rd day.Meanwhile the more heavy inflammation was always found in nude mice than BALB/c mice. Conclusions ：The similar RSV infectious characteristic exists between BALB/c mice and nude mice. But the more severe infection happens in nude mice, which benefits to assess the effect of anti-RSV drugs and is ideal mice model of RSV infection.

关 键 词：呼吸道合胞病毒 小鼠 动物模型 

分 类 号：R373.1[医药卫生—病原生物学]