丙型肝炎病毒持续感染对体外培养人胎盘合体滋养层细胞MMP-2和MMP-9合成分泌的影响  

Effects of HCV persistent infection on synthesis and secretion of MMP-2 and MMP-9 of human placental syncytiotrophoblast in vitro

作　　者：张亚飞[1] 聂青和[1] 邵彬[1] 罗新栋[1] 王平忠[1] 陈朝霞[1] 徐辉[2] 李军[1] 

机构地区：[1]第四军医大学唐都医院全军感染病诊疗中心,陕西西安710038 [2]第四军医大学唐都医院消化内科,陕西西安710038

出　　处：《第四军医大学学报》2006年第21期1949-1952,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(39970767)

摘　　要：目的:探讨丙型肝炎病毒持续感染对体外培养人胎盘合体滋养层细胞MMP-2和MMP-9合成分泌的影响.方法:应用ELISA方法检测细胞培养上清MMP-2和MMP-9水平,分析感染组和对照组数据间的差异,并用明胶酶谱进一步验证ELISA结果,随后应用RT-PCR法分析感染对细胞proM-MP-2和proMMP-9mRNA表达的影响.结果:感染组细胞分泌MMP-2和MMP-9能力明显下降(MMP-2:t=4.186,P<0.05;MMP-9:t=2.325,P<0.05);RT-PCR结果显示感染组细胞proMMP-2和proMMP-9mRNA表达弱于对照组,但差异不明显(proMMP-2:t=1.196,P>0.05;proMMP-9:t=1.417,P>0.05).结论:丙型肝炎病毒持续感染时体外培养人胎盘合体滋养层细胞MMP-2和MMP-9合成分泌能力下降,为非特异性.AIM： To investigate the effect of HCV persistent infection on synthesis and secretion of MMP-2 and MMP-9 of human placental syncytiotrophoblast in vitro. METHODS： ELISA was employed to evaluate the effect of HCV persistent infection on secretion of MMP-2 and MMP-9 of human placental syncytiotrophoblast in vitro, and furthermore, gelatin zymography was used to validate the ELISA result. The effect of HCV persistent infection on synthesis of proMMP-2 and proMMP-9 mRNA was analyzed by RT-PCR. RESULTS： The secretion of MMP-2 and MMP-9 of human placental syncytiotrophoblast in infection group decreased significantly （ MMP-2 ： t = 4. 186, P 〈 0.05 ; MMP-9 ： t = 2.325, P 〈 0.05） ; The synthesis of proMMP-2 and proMMP- 9 mRNA of syncytiotrophoblast of infection group decreased in comparison with control group, but not significantly in statistics （proMMP-2： t = 1. 196, P 〉 0.05; proMMPo9： t = 1. 417, P 〉 0.05 ）. CONCLUSION ： HCV persistent infection suppressed the synthesis and secretion of MMP-2 and MMP-9 of human placental syncytiotrophoblast in vitro, which is not specific.

关 键 词：丙型肝炎病毒 合体滋养层细胞 金属蛋白酶-2 -9 体外 

分 类 号：R512.63[医药卫生—内科学]