从土壤中分离的棘阿米巴属CJY/S1和CJY/S2株的18S rDNA基因型鉴定  

Genetic Identification of Acanthamoeba sp. CJY/S1 and CJY/S2 Isolated from Soil

作　　者：郑善子[1] 玄英花[1] 王月华[2] 申成华[1] 崔春权[1] 

机构地区：[1]延边大学基础医学院免疫学与病原生物学教研部,延吉133000 [2]吉林医药学院病原生物学教研室,吉林132013

出　　处：《中国寄生虫学与寄生虫病杂志》2006年第5期391-392,共2页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：延边大学科技发展计划项目(No.2004-36)~~

摘　　要：从吉林延边地区土壤中分离的棘阿米巴属CJY/S1和CJY/S2株中提取基因组18SrDNA,PCR扩增、克隆、测序后用分子生物学软件ClustalX进行序列分析,与基因库中已有T1至T12型序列进行比较并构建进化树。结果棘阿米巴土壤分离株Acanthamoebasp.CJY/S1和CJY/S株的18SrDNA全基因序列分别为2255bp和2252bp,均属T4基因型。Two isolates of Acanthamoeba sp. CJY/S1 and CJY/S2 were received from soil in Yanji of Jilin Province. Full 18S rDNA gene was amplified using PCR, eloned and sequenced. The results were analyzed by software Clustal X. The full length of CJY/S1 and CJY/S2 is 2255 bp and 2252 bp respectively, both belong to T4 genotype.

关 键 词：棘阿米巴 18S RDNA 基因型 分类 

分 类 号：R382.1[医药卫生—医学寄生虫学]