实时PCR定量分析干旱胁迫下水稻糖原合成酶激酶基因表达差异  

Study on Glycogen Synthase Kinase Gene Expression Variation under Drought Stress in Rice by Real-Time PCR

作　　者：魏敏[1] 熊建华[1] 李阳生[1] 傅彬英[2] 

机构地区：[1]武汉大学生命科学学院植物发育生物学教育部重点实验室,湖北武汉430072 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《中国水稻科学》2006年第6期567-571,共5页Chinese Journal of Rice Science

基　　金：国家973计划资助项目(2001CB1008-05;2003CB114308);创新研究群体科学基金资助项目(30521004);长江学者和创新团队发展计划资助项目(PCSIRT)

摘　　要：通过实时荧光定量PCR进行相对定量分析,得到水稻IR64糖原合成酶激酶(glycogen synthase kinase,GSK)基因在不同生育期、不同组织干旱胁迫处理前后的表达差异。叶片中GSK基因为组成型表达,各生育期干旱胁迫时诱导合成增加,尤其从分蘖期到抽穗期表达更为显著,说明这段生长期叶片对水分缺乏最敏感;苗期根系处理后GSK表达明显下降,叶片处理前后GSK表达无明显变化。由此可见,GSK基因的表达在不同的发育时期、不同的组织中呈现差异。Glycogen synthase kinase 3/shaggy-like （GSK） genes encode kinases that are involved in a variety of biological activities. To study the differential expression of GSK gene under drought stress at different developmental stages and in different tissues of rice variety IR64, relatively quantitative analysis was performed by real-time PCR. The expression level of GSK gene in leaf was higher under drought stress than under the water treatment, and the increase was especially obvious from the tillering to heading stages, which suggested that rice leaves are very sensitive to water shortage at these developmental stages. However, the expression level of GSK gene in root at the seedling stage decreased notably under drought stress whereas kept stable in leaf. Therefore, it is considered that GSK gene of rice expressed differently at different growth stages and in different tissues.

关 键 词：水稻 糖原合成酶激酶 干旱胁迫 实时PCR 相对定量分析 

分 类 号：S511[农业科学—作物学]