一个丙环唑诱导的水稻基因cDNA片段的分离与分析  

Identification of a Propiconazol Inducible Gene in Rice

作　　者：殷得所[1] 王世全[1] 邓其明[1] 李双成[1] 王玲霞[1] 李平[1] 

机构地区：[1]四川农业大学水稻研究所

出　　处：《中国水稻科学》2006年第6期572-576,共5页Chinese Journal of Rice Science

基　　金：国家863计划资助项目(2003AA212030);教育部长江学者和创新团队发展计划资助项目(IRT0453)

摘　　要：采用差异显示技术,以丙环唑作为诱导剂,对水稻幼苗进行筛选,得到一个化学诱导表达增强的差异片段。生物信息学分析表明,此片段可在水稻EST库中找到多个同源序列,其氨基酸序列与水稻、拟南芥等植物的GMPase基因高度同源,位于水稻第1染色体,是抗坏血酸合成途径中的关键酶。RT-PCR实验表明此基因在丙环唑诱导后表达增强。After rice seedlings were induced by spraying the fungicide propiconazole, a cDNA fragment was cloned by mRNA differential display, whose corresponding transcription was enhanced in response to chemical treatment. Bioinformaties analysis revealed that this cDNA was homologous to multiple ESTs of rice and its amino acid sequences was homologous to GMPase gene in several plants including rice on chromosome 1 with 100% identities. RT-PCR showed that the transcript level increased after application of exogenous propiconazole.

关 键 词：水稻 化学诱导 MRNA差异显示 鸟苷-甘露糖焦磷酸化酶基因 丙环唑 

分 类 号：S511[农业科学—作物学]