水稻愈伤组织中脱落酸特异诱导蛋白的纯化及分析  

Purification and Analysis of Abscisic Acid-Specifically-Inducible Protein from Rice Callus

作　　者：姜华[1] 万佳[1] 高晓玲[1] 陈静[1] 汪旭东[1] 徐正君[1] 

机构地区：[1]四川农业大学水稻研究所

出　　处：《中国水稻科学》2006年第6期583-588,共6页Chinese Journal of Rice Science

基　　金：四川省国际合作项目(05GH0001);教育部长江学者和创新团队发展计划(IRT0453);四川省青年科技基金资助项目

摘　　要：用硫酸铵分级沉淀、离子交换(DEAE-Sepharose和TSK-gel)的方法,结合SDS-PAGE和2D电泳(two-dimension electrophoresis)技术,从水稻愈伤组织中分离并精制了两个分子量相同(24.5 kD),等电点分别为6.1和6.9的脱落酸(ABA)特异诱导蛋白。Western-blot印迹分析发现,这两个蛋白在不同组织中的表达差异明显。A1(pI 6.1)蛋白在ABA处理的水稻愈伤组织和种子胚中表达,但在未经ABA处理的材料(包括幼芽、愈伤组织)和白色干燥性愈伤组织中没有检测到表达;A2(pI 6.9)蛋白在经ABA处理材料、种胚和白色干燥性愈伤组织中检测到表达,在未经ABA处理的材料中没有检测到表达。推测这两个蛋白可能在胚(或不定胚)的形成过程中起重要作用。Two ABA-specifically-inducible proteins from rice callus, were isolated and purified by precipitation with 65 --100% saturated （NH4）2 SO4 , followed by the DEAE-sepharose, TSK-gel, and two-dimension electrophoresis. Iso-electric point （pI） of the proteins with the same molecular mass （24.5 kD）were 6.1 and 6.9 respectively. Western blotting analysis indicated that the expression of the proteins at the different tissues had obvious diversity. The A1 （ pI 6. 1） protein was detected in the rice callus treated with ABA and the seed embryos （SE）, but not in young plants, the callus without ABA treatment and the white dry callus （WDC）. The A2 （pI 6.9） protein was found in the callus treated with ABA, WDC and SE, but not in other materials without ABA treatment. These two proteins might play some important roles in the processes of seed embryo （or somatic embryos） formation.

关 键 词：水稻 愈伤组织 脱落酸 特异诱导蛋白 纯化 表达 

分 类 号：S511[农业科学—作物学]