DNA与两性表面活性剂相互作用研究  

The Interaction between DNA and Amphoteric Surfactants

作　　者：孙彦庆[1] 张剑[2] 张高勇[1] 王红霞[1] 

机构地区：[1]中国日用化学工业研究院表面活性剂国家工程研究中心,太原030001 [2]太原理工大学煤科学与技术教育部和山西省重点实验室,太原030032

出　　处：《化学进展》2006年第11期1440-1445,共6页Progress in Chemistry

基　　金：国家自然科学基金项目资助(No.20233010)

摘　　要：本文综述了DNA与两性表面活性剂相互作用的研究进展,主要介绍了利用荧光显微镜、动静态光散射、相图及浊度等方法对DNA与两性表面活性剂相互作用的观察与形成复合物的表征。由于两性表面活性剂所具有的独特性质,可以实现在特定pH范围通过静电作用诱导DNA构象发生线圈状向小球状的不连续转变,并可通过调节溶液pH、离子强度等实现对DNA-两性表面活性剂复合物的稳定性的调控。DNA与两性表面活性剂相互作用形成的复合物在非病毒基因载体研究方面具有潜在的应用价值。The research progress of interaction between DNA and amphoteric surfactant is reviewed. Relevant studying methods of interaction-observering and complex-charactering, such as fluorescent microscopic technology, light scattering, phase diagram and turbidimetric titration are expatiated. Owing to the characteristic of amphoteric surfactant, in specific range of pH it could induce discrete conformations] transition of DNA from unfolded coil to compacted globule by electrostatic attraction. Interaction between DNA and amphoteric surfactant is controlled by pH and ion strength. The DNA-amphoteric surfactant complexes have potential application as gene vectors.

关 键 词：DNA 两性表面活性剂 相互作用 基因载体 

分 类 号：O647.2[理学—物理化学] Q523[理学—化学]