氟化钠对放免分析血浆胰岛素的影响  

Effect of NaF on RIA for plasma insulin

作　　者：夏凤城[1] 汪寅章[1] 

机构地区：[1]解放军总医院内分泌科

出　　处：《军医进修学院学报》1996年第4期274-276,共3页Academic Journal of Pla Postgraduate Medical School

摘　　要：采用液相和固相胰岛素放免分析法，观察氟化钠（ＮａＦ）抗凝剂对血浆胰岛素测定结果的影响，并与肝素抗凝剂作比较。液相放免测定的ＮａＦ抗凝血浆胰岛素水平比对照血清组低３８．７％；固相放免法在低浓度胰岛素样品（≤２０ｍＵ／Ｌ）中，ＮａＦ抗凝血浆高于血清组３１．７％，而高浓度胰岛素样品（＞２０ｍＵ／Ｌ）的ＮａＦ抗凝血浆反而低于血清组１０．６％。结果差异有极显著性意义（Ｐ＜０．０１～０．００１）。肝素抗凝血浆胰岛素水平与血清差异无显著性意义（Ｐ＞０．０５）。结果提示：ＮａＦ抗凝剂对胰岛素放免测定有明显影响，而且在液相与固相放兔反应中表现不同，不能用单一措施纠正，其机制还有待于进一步探讨。因此，ＮａＦ不适用于胰岛素血样的抗凝剂。Although sodium fluoride(NaF)is a good anticoagulant for plasma glucose measurement,it has not been known yet whether NaF could be used as an anticoagulant in RIA for plasma insulin.We observed the effect of NaF on RIA for plasma insulin by the use of liquid-phase and solid-phase RIA,and cotnpared NaF with heparin as anticoagulant.It was found that in liquid-phase RIA the insulin level of plasrna with NaF de-creased by 38.7%vs that of serum;however in the solid-phase RIA the insulin level of plasrna with NaF var-ied with insulin concentration in samples:the plasma insulin level increased by 31.7%in the satnples of low insulin concentration(≤20 mU/L)and decreased by 10.6% in the high insulin concentration samples(>20 mU/L)vs those in the rnatched serum samples.There was no difference in insulin levels between plasma spec-imen with heparin and serurn sample either by liquid-phase or solid-phase RIA. It was evident that NaF could interfere with RIA for plasrna insulin,although its mechanism has not been clear it will not be fit for an anti-coagulant in plasma insulin RIA.

关 键 词：糖尿病 氟化钠 胰岛素 放免分析 

分 类 号：R587.104[医药卫生—内分泌] R446.112[医药卫生—内科学]