Breeding of Selectable Marker-Free Transgenic Rice Lines Containing AP1 Gene with Enhanced Disease Resistance  

作　　者：YU Heng-xiu LIU Qiao-quan WANG Ling ZHAO Zhi-peng XU Li HUANG Ben-li GONG Zhi-yun TANG Shu-zhu GU Ming-hong 

机构地区：[1]State Key Laboratory of Plant Functional Genomics, Ministry of Education/Yangzhou University, Yangzhou 225009, P.R.China [2]China National Rice Research Institute, Hangzhou 310006, P.R.China [3]Department of Plant Protection, Agricultural College, Yangzhou University, Yangzhou 225009, P.R.China

出　　处：《Agricultural Sciences in China》2006年第11期805-811,共7页中国农业科学（英文版）

基　　金：This paper is translated from its Chinese version in Scientia Agricultura Sinica.This study was supported by the Government of Jiangsu Province,China(BG2002301 and JH02-106);National Transgenic Plant R&D Project(JY03-B-10);National Natural Science Foundation of China(30170567);Department of Education of Jiangsu Goverment,China(K05015).

摘　　要：In order to obtain marker-free transgenic rice with improved disease resistance, the AP1 gene of Capsicum annuum and hygromycin-resistance gene （HPT） were cloned into the two separate T-DNA regions of the binary vector pSB130, respectively, and introduced into the calli derived from the immature seeds of two elite japonica rice varieties, Guangling Xiangjing and Wuxiangjing 9, mediated by Agrobacterium-mediated transformation. Many cotransgenic rice lines containing both the AP1 gene and the marker gene were regenerated and the integration of both transgenes in the transgenic rice plants was confirmed by either PCR or Southern blotting technique. Several selectable marker-free transgenic rice plants were subsequently obtained from the progeny of the cotransformants, and confirmed by both PCR and Southern blotting analysis. These transgenic rice lines were tested in the field and their resistance to disease was carefully investigated, the results showed that after inoculation the resistance to either bacterial blight or sheath blight of the selected transgenic lines was improved when compared with those of wild type.In order to obtain marker-free transgenic rice with improved disease resistance, the AP1 gene of Capsicum annuum and hygromycin-resistance gene （HPT） were cloned into the two separate T-DNA regions of the binary vector pSB130, respectively, and introduced into the calli derived from the immature seeds of two elite japonica rice varieties, Guangling Xiangjing and Wuxiangjing 9, mediated by Agrobacterium-mediated transformation. Many cotransgenic rice lines containing both the AP1 gene and the marker gene were regenerated and the integration of both transgenes in the transgenic rice plants was confirmed by either PCR or Southern blotting technique. Several selectable marker-free transgenic rice plants were subsequently obtained from the progeny of the cotransformants, and confirmed by both PCR and Southern blotting analysis. These transgenic rice lines were tested in the field and their resistance to disease was carefully investigated, the results showed that after inoculation the resistance to either bacterial blight or sheath blight of the selected transgenic lines was improved when compared with those of wild type.

关 键 词：rice （Oryza sativa L.） disease resistance AP1 gene selectable marker-free bacterial blight sheath blight 

分 类 号：S511[农业科学—作物学]