ISSR Marker and ITS Sequence Study of Melampsora Larici-populina  

作　　者：YU Zhong-dong LIU Xiao-yong CAO Zhi-min 

机构地区：[1]College of Forestry, Northwest A＆F University, Yangling 712100, P.R.China [2]Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P.R. China

出　　处：《Agricultural Sciences in China》2006年第11期847-854,共8页中国农业科学（英文版）

基　　金：This paper is translated from its Chinese version in Scientia Agricultura Sinica.This study is financially supported by National Natural Science Foundation of China(30471394);Natural Science Foundation of Shaanxi Province,China(ShaanC113).

摘　　要：To compare the differences in intertranslation space of ribosomal DNA （ITS） of Melampsora larici-populina, between the isolates from China and isolates from other countries, this study investigated ITS sequences and ITS polygenetic tree based on 11 isolates that were collected from 5 races in different parts of China. The results indicated that there was no difference among the ITS sequences of 11 isolates from China. The ITS sequence of isolates from China was more homogeneous with that of isolates from Britain compared with France, Germany, and Canada. Intersimple sequence repeat （ISSR） markers were also used to study the genetic division of Melampsora larici-populina, and the results showed that the 11 tested isolates could be divided into Western population and Northern population. Genetic diversity index of race C2 was significantly different from that of races C4, C3, and C1, and no significant differences were observed among the other races. Pathogenicity division of races must not harmonize with their genetic division, except race C2. The ITS region is conservative, and ITS sequence is not fit for studying the differences that existed among the races. ISSR marker can be used for intraspecies population study, and Melampsora larici-populina in China can be divided into two populations.To compare the differences in intertranslation space of ribosomal DNA （ITS） of Melampsora larici-populina, between the isolates from China and isolates from other countries, this study investigated ITS sequences and ITS polygenetic tree based on 11 isolates that were collected from 5 races in different parts of China. The results indicated that there was no difference among the ITS sequences of 11 isolates from China. The ITS sequence of isolates from China was more homogeneous with that of isolates from Britain compared with France, Germany, and Canada. Intersimple sequence repeat （ISSR） markers were also used to study the genetic division of Melampsora larici-populina, and the results showed that the 11 tested isolates could be divided into Western population and Northern population. Genetic diversity index of race C2 was significantly different from that of races C4, C3, and C1, and no significant differences were observed among the other races. Pathogenicity division of races must not harmonize with their genetic division, except race C2. The ITS region is conservative, and ITS sequence is not fit for studying the differences that existed among the races. ISSR marker can be used for intraspecies population study, and Melampsora larici-populina in China can be divided into two populations.

关 键 词：Melampsora larici-populina RACE ITS sequence ISSR marker 

分 类 号：S43[农业科学—农业昆虫与害虫防治]