钆对费氏中华根瘤菌抑制效应的蛋白质组分析  

Proteome Analysis of Inhibitory Effect of Gadolinium on Sinorhizobium Fredii

作　　者：韩威威[1] 沈世华[1] 台培东[2] 

机构地区：[1]中国科学院植物研究所光合作用与环境分子生理学重点实验室 [2]中国科学院沈阳应用生态研究所,辽宁沈阳110016

出　　处：《中国稀土学报》2006年第5期623-627,共5页Journal of the Chinese Society of Rare Earths

基　　金：国家重点基础研究发展规划资助项目(G2001CB108902;2004CB418506)

摘　　要：运用双向凝胶电泳和MALDI-TOF质谱技术,分析了Gd3+对费氏中华根瘤菌USDA 205的抑制效应。结果表明,经1 mmol.L-1Gd3+处理12 h后,22个蛋白质表达量有显著变化,其中13个蛋白质表达量增加,9个蛋白质表达量下降。这些蛋白质可根据功能分为8类,包括转运蛋白、胁迫相关蛋白、代谢相关蛋白等。The inhibitory effect of gadolinium on Sinorhizobium fredii USDA 205 was globally studied through two gel dimensional electrophoresis and MALDI-TOF MS. The results indicate that 22 proteins are significantly affected under 1 mmol·L^- Gd^3＋ treatment when compared to an untreated control. Among these proteins, nine are up-regulated and thirteen are down-regulated. The differently expressed proteins are classified into 8 functional categories based on their functions, including transporters, proteins for cellular defense, proteins involved in metabolism.

关 键 词：费氏中华根瘤菌 钆 蛋白质组 稀土元素 

分 类 号：O614.3[理学—无机化学]