一种用于食草动物粪便显微组织分析的临时装片新技术  

A new technique for temporary slide mounting in microhistological herbivore fecal analysis

作　　者：曹伊凡[1] 苏建平[1] 

机构地区：[1]中国科学院西北高原生物研究所,西宁810001

出　　处：《兽类学报》2006年第4期407-410,共4页Acta Theriologica Sinica

基　　金：青海省重大科技攻关项目(2002-N-105);中国科学院知识创新工程领域前沿项目(CXLY-2002-3)

摘　　要：粪便显微组织分析法是研究食草动物食性的主要方法,其常规装片技术需要使用Hoyer’s装片介质对植物碎片进行封片,而Hoyer’s封片液的粘性易导致植物碎片在装片过程中发生卷曲和重叠,影响植物碎片的识别效果。本文提出的新装片技术采用没有粘性的饱和NaCl溶液代替Hoyer’s装片介质,结合特定的定量取样方法和装片程序,可以有效地减少植物碎片的卷曲率和重叠率。对比试验显示,新装片技术可使植物碎片卷曲率从10.4%下降至3.8%,重叠率从25%下降至8.1%,说明新装片技术在减少植物碎片卷曲和重叠方面明显优于常规装片方法。Microhistological fecal analysis is an important technique for investigating herbivore diets. The conventional mounting technique uses Hoyer＇ s mounting medium to seal the plant fragment to the slide. However, Hoyer＇ s seal liquid inevitably causes plant fragments to crinkle and overlap during the mounting process because of the liquid＇ s stickiness, which leads to a poor identification of sample plant fragments. To reduce this problem, we developed a new mounting technique to replace Hoyer＇ s mounting medium： non-viscous saturated NaCI. We performed a controlled experiment in which ten slides of the same feces sample were made and observed, using new and the conventional mounting technique. The percentage of crinkled fragments declined frdm 10. 4% （ conventional mounting technique ） to 3.8% （ new mounting technique） and of overlapping fragments from 25% （conventional mounting technique） to 8. 1% （ new mounting technique）. We recommend the new mounting technique over the conventional mounting technique.

关 键 词：食草动物食性 显微组织分析 植物碎片 装片技术 

分 类 号：Q336[生物学—遗传学]