Molecular analysis of hepatitis B virus isolates in Mexico:Predominant circulation of hepatitis B virus genotype H  

作　　者：Cosme Alvarado-Esquivel Erwin Sablon Carlos Jesús Conde-GonzálezNational Institute of Public Health  Cuernavaca  Morelos  Mexico Luis Juárez-Figueroa Lilia Ruiz-Maya Sergio-Aguilar Benavides 

机构地区：[1]Faculty of Medicine, Universidad Juárez del Estado de Durango, Durango, Dgo, Mexico [2]Innogenetics NV Ghent, Belgium [3]National Institute of Public Health, Cuernavaca, Morelos, Mexico [4]Regional Laboratory of Epidemiologic Reference, Guadalajara, Jal, Mexico

出　　处：《World Journal of Gastroenterology》2006年第40期6540-6545,共6页世界胃肠病学杂志（英文版）

摘　　要：AIM: To determine the genotypes in Mexican hepatitis Bvirus (HBV) isolates and characterize their precore andcore promoter mutations.METHODS: Forty-nine HBV isolates of Mexico obtainedfrom sera of 15 hepatitis patients, 6 hemodialysis pa-tients, 20 men seeking HIV testing, and 8 AIDS patientswere analyzed. HBV isolates were amplified by PCR,and genotyped by line probe assay (INNO-LiPA HBVGenotyping; INNOGENETICS N V, Ghent, Belgium).HBV genotype confirmation was performed by DNAsequencing part of the sAg region. Precore and core pro-moter mutation characterization was performed by lineprobe assay (INNO-LiPA HBV PreCore; INNOGENETICS NV, Ghent, Belgium).RESULTS: Overall, HBV genotype H was found in 37(75.5%) out of the 49 isolates studied. HBV genotypesG, A, and D were found in 5 (10.2%), 4 (8.2%), and 3(6.1%) isolates, respectively. HBV genotype H was pre-dominant in isolates from hemodialysis patients (100%),hepatitis patients (80%), and men seeking HIV test-ing (75%), and accounted for half of infections in AIDSpatients (50%). Six (12.2%) out of the 49 HBV isolatesshowed both wild type and mutant populations at pre-core codon 28. These mixed wild type and precore mu-tant populations were observed in one HBV genotype Aisolate and in all HBV genotype G isolates. A dual variantcore promoter mutation was observed in 1 (2%) of theisolates, which was genotype H.CONCLUSION: HBV genotype H is highly predominantin HBV isolates of Mexico followed by genotypes G, Aand D. A low frequency of precore and core promotermutations is observed in HBV Mexican isolates.AIM： To determine the genotypes in Mexican hepatitis B virus （HBV） isolates and characterize their precore and core promoter mutations. METHODS： Forty-nine HBV isolates of Mexico obtained from sera of 15 hepatitis patients, 6 hemodialysis patients, 20 men seeking HIV testing, and 8 AIDS patients were analyzed. HBV isolates were amplified by PCR, and genotyped by line probe assay （INNO-LiPA HBV Genotyping; INNOGENETICS N V, Ghent, Belgium）. HBV genotype confirmation was performed by DNA sequencing part of the sAg region. Precore and core promoter mutation characterization was performed by line probe assay （INNO-LiPA HBV PreCore; INNOGENETICS N.V.; Ghent, Belgium）. RESULTS： Overall, HBV genotype H was found in 37 （75.5%） out of the 49 isolates studied. HBV genotypes G, A, and D were found in 5 （10.2%）, 4 （8.2%）, and 3 （6.1%） isolates, respectively. HBV genotype H was predominant in isolates from hemodialysis patients （100%）, hepatitis patients （80%）, and men seeking HIV testing （75%）, and accounted for half of infections in AIDS patients （50%）. Six （12.2%） out of the 49 HBV isolates showed both wild type and mutant populations at precore codon 28. These mixed wild type and precore mutant populations were observed in one HBV genotype A isolate and in all HBV genotype G isolates. A dual variant core promoter mutation was observed in 1 （2%） of the isolates, which was genotype H. CONCLUSION： HBV genotype H is highly predominant in HBV isolates of Mexico followed by genotypes G, A and D. A low frequency of precore and core promoter mutations is observed in HBV Mexican isolates.

关 键 词：Hepatitis B virus GENOTYPES Mutations Molecular epidemiology 

分 类 号：R373[医药卫生—病原生物学]