白菜病程相关蛋白1基因cDNA全长的克隆与分析  

Cloning and Characterization of a Full-length cDNA of Pathogenesis-related Protein 1 in Brassica rapa ssp.chinensis

作　　者：王彦华[1] 侯喜林[1] 申书兴[2] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095 [2]河北农业大学园艺学院,河北保定071001

出　　处：《园艺学报》2006年第5期985-988,共4页Acta Horticulturae Sinica

基　　金：高等学校博士点科研基金项目(20030307021)

摘　　要：以水杨酸处理后的‘苏州青’白菜为材料，通过RT—PCR和RACE技术，获得了白菜病程相关蛋白PR-1基因的cDNA全长序列。该基因与甘蓝型油菜PR-1基因氨基酸序列的同源性为91％，与拟南芥的同源性为78％，与其它植物的同源性为57％～60％。Southern杂交表明该基因在白菜基因组中的拷贝数至少为2个。半定量RT—PCR分析表明，2mmol／L的水杨酸处理后12h，该基因的表达量达到高峰。In this study, according to the consensus domain of the Brassica napus pathogenesis-related protein PR-1 gene （accession number U21849）, using RT-PCR and RACE technology, a full-length cDNA of pathogenesis-related protein 1 named BcPR-1 was obtained from Brassica rapa ssp. chinensis cultivar Suzhouqing, which displayed resistance to downy mildew. Sequence analysis indicated that cloned BcPR-1 gene consisted of 646 nucleotides （nt） containing 161 amino acids. Further comparison to B. napus PR-1 gene and Arabidopsis thaliana PR-1 gene showed that its identity was 91% and 78%, respectively. Its similarity to other PR-1 genes was 57% to 60%. Southern-blot analysis indicated that there were at least two copies of BcPR-1 gene in B. rapa ssp. chinensis genome. Semi-quantitative RT-PCR analysis revealed that expression of BcPR-1 gene was induced by SA treatment in Suzhouqing, and the corresponding mRNA was accumulated most abundandy 12 h after treatment upon 2 mmol/L SA.

关 键 词：白菜 病程相关蛋白1 水杨酸 RT—PCR 

分 类 号：S634.3[农业科学—蔬菜学]