番茄转ER-sHSP基因植株构建及其抗冷性研究  

The Construction and the Chilling-Resistance Ability of Endoplasmic Reticulum Small Heat Shock Protein (ER-sHSP) Transgenic Tomato Plants

作　　者：赵春梅[1] 王丽[1] 伊淑莹[1] 刘箭[1] 

机构地区：[1]山东师范大学生命科学学院,山东济南250014

出　　处：《园艺学报》2006年第5期989-994,共6页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30270132)

摘　　要：将CaMV35S启动子驱动的内质网小分子热激蛋白基因导入番茄,比较转基因、未转基因和转空载体番茄的抗冷能力。冷胁迫下,同对照相比,转基因番茄的冷害症状轻,叶绿素含量的损失少,体内积累的丙二醛(MDA)含量少,电解质外渗程度低,具有较高的净光合速率和最大光化学效率,并易于恢复由低温所引起的光抑制,表明转基因番茄具有较强的冷胁迫耐性,说明内质网小分子热激蛋白在植物抗冷过程中发挥重要作用。The full-length endoplasmic reticulum small heat shock protein （ER-sHSP） cDNA using CaMV 35S promoter was transformed into the genome of tomato plants. The chilling tolerance of transgenic tomato plants, non-transgenic tomato plants and pROK Ⅱ-transformed tomato plants were studied. Under chilling stress treatment, compared with non-transgenic and pROK Ⅱ-transformed tomato plants, transgenic tomato plants exhibited slighter cold-injured symptoms, suffered less destruction of chlorophyll and electrolyte leakage. The content of MDA of transgenic tomato plants was lower than that of non-transgenic and pROK Ⅱ -transformed tomato plants, and transgenic tomato plants could keep up higher value of net photosynthetic rate, Fv/ Fm and could recover quickly from chilling-induced photosynthetic inhibition. The results showed that ER- sHSP played a key role in enhancing the chilling-resistance ability of plants.

关 键 词：番茄 内质网 小分子热激蛋白 转基因 冷胁迫 

分 类 号：S641.2[农业科学—蔬菜学]