TuMV-Nib反义基因对大白菜的遗传转化研究  

Genetic Transformation of Anti-TuMV-Nib into Transgenic Chinese Cabbages

作　　者：于占东[1] 赵双宜[2] 何启伟[1] 王翠花[1] 牟晋华[1] 刘春香[1] 康静[3] 

机构地区：[1]山东省农业科学院蔬菜研究所,山东济南250100 [2]山东大学生命科学院,山东济南250100 [3]吉林农业大学园艺学院,吉林长春130118

出　　处：《园艺学报》2006年第5期1093-1095,共3页Acta Horticulturae Sinica

基　　金：山东省自然科学基金资助项目(Y2004D02);吉林农业大学科研基金资助项目

摘　　要：以‘福山包头’大白菜子叶为试材,以Anti-TuMV-Nib为目的基因,利用根癌农杆菌介导法,建立了大白菜再生转化体系,获得转化植株。经PCR-Southern杂交、RT-PCR及ELISA检测,证明外源TuMV-Nib基因已整合到大白菜核基因组中并获得了表达。Agrobacterium tumiefaciens-mediated regeneration system was established using Anti-TuMV- Nib gene. Cotyledons with petiole from aseptic seedlings of Chinese cabbage （Brassica campestris ssp. pekinensis ‘Fushanbaotou＇ ） were used as explants and the transgenic plants were obtained. The analysis of PCR- Southern blotting, RT-PCR and ELISA detection showed that the target gene was integrated into the Chinese cabbage genome and expressed.

关 键 词：大白菜 芜菁花叶病毒 复制酶基因 遗传转化 

分 类 号：S634.1[农业科学—蔬菜学]