海马提取物调控PI4-K激酶活性  

Regulatory effects of hippocampus extracts on PI4-K activity

作　　者：陈芳[1] 张璠[1] 吕会田[2] 乐加昌[1] 殷勤伟[1] 张偲[3] 

机构地区：[1]中国科学院生物物理研究所生物大分子国家重点实验室 [2]北京工商大学化学与环境工程学院,北京100037 [3]中国科学院南海海洋研究所,广东广州510301

出　　处：《基础医学与临床》2006年第11期1210-1214,共5页Basic and Clinical Medicine

基　　金：国家高技术研究发展计划项目(2002AA629150)

摘　　要：目的海马是一种具有抗衰老、抗疲劳及抗肿瘤的中药,迄今对其分子作用机制不十分清楚。肌醇磷脂激酶(PI4-K)作为PI信号通路中起重要作用的激酶,位于信号通路的上游,应是寻找新药十分关键的靶分子。方法利用人红细胞膜内翻外的技术,将存在于细胞膜内侧的肌醇磷脂激酶(PI4-K)暴露在外,成为IOV-PI4-K抗原蛋白;用IOV-PI4-K抗原蛋白与其PI4-K单克隆抗体(A6D)的ELISA检测技术,对海马提取物进行了初步研究。结果从海马提取物中筛选出了对PI4-K与抗体活性亲和力具有上调或下调作用的两种不同组分,提示在这二种组分里可能存在有不同的分子对PI4-K有着上调或下调的作用。结论从上述二种组分里有可能开发出治疗肿瘤和抗衰老的药物。这一筛选方法也可为今后的药物筛选提供一种新的思路。Objective The hippocampus has been used in Chinese medicine for a long time. Although it has the anti-decrepitude, anti-fatigue and anti-tumor effect, the molecular mechanism is not yet known. PI4-K, the first protein kinase in the PI pathway, should be the target molecular for drug screening. Methods We prepared antigen protein IOV-PI4-K through inside-out ghost, then the antibody-antigen reaction between IOV-PI4-K and its antibody A6D was tested by ELISA. Results Two different function groups, which could increase or decrease the binding affinity of PI4-K to its antibody, were screened out from the extracts of hippocampus. This fact indicated that there might be two different components to up-regulate or down-regulate the activity of PI4-K respectively. Conclusion Derivatives of the components could be developed into anti-tumor or anti- decrepitude drugs. Meanwhile, a new way for performing drug screen through ELISA was established.

关 键 词：肌醇磷脂激酶PI4-K 海马提取物 间接ELISA 人红细胞膜内翻外 药品筛选 

分 类 号：R341[医药卫生—基础医学]